RESULTS:
1 - 20 of 41 for "Feng-Li Hui"
Pseudobensingtonia carpini sp. nov., a novel yeast species isolated from plant leaves in China
Two novel yeast strains NYNU 236247 and NYNU 23523 were isolated from the leaves of Carpinus turczaninowii Hance collected in the Tianchi Mountain National Forest Park Henan Province central China. Phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region revealed the closest relatives of the strains are three described Pseudobensingtonia species: Ps. fusiformis Ps. musae and Ps. ingoldii. The novel species differed from the type strains of these three species by 12 to 22 nucleotide substitutions and 1 gap (~2.0–4.0%) in the D1/D2 domain and by 78 to 100 nucleotide mismatches (~12.0–16%) in the ITS region. Physiologically the novel species differs from Ps. fusiformis and Ps. musae in its ability to assimilate dl-lactate and melezitose and from Ps. ingoldii by its inability to assimilate melibiose soluble starch and ethanol. Pseudobensingtonia carpini sp. nov. is proposed for those two strains with the holotype designated as GDMCC 2.483T (MycoBank MB 857072).
Meira qingyuanensis sp. nov., an anamorphic yeast species isolated from plant leaves
Two yeast strains NYNU 224191 and NYNU 22411 were isolated from plant leaves of Cyclobalanopsis jenseniana and Schisandra sp. harvested from Qingyuan Mountain Quanzhou City Fujian Province southeast China. Molecular phylogenetic analyses demonstrated that these two strains represent a novel species in the genus Meira. They are more closely related to M. marina M. nashicola M. plantarum and M. pileae than other Meira species based on phylogenetic analysis of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit (LSU) rRNA gene. The novel species differed from its closest relatives M. marina M. nashicola M. plantarum and M. pileae by 1.8–2% sequence divergence (11–12 substitutions and 1–2 gaps) in the D1/D2 domain and over 6.7% sequence divergence (40–60 substitutions and 53–77 gaps) in the ITS region respectively. We propose the name Meira qingyuanensis sp. nov. (Holotype CICC 33567; Mycobank MB 855328) for the new species which differs phenotypically from M. marina M. plantarum and M. pileae in its ability to assimilate methyl-α-d-glucoside and the inability to assimilate salicin and from M. nashicola in its ability to assimilate nitrate and nitrite and grow at 30 °C.
Naohidea akebiae fa. sp. nov., an anamorphic basidiomycete yeast species
A novel basidiomycete yeast species represented by strain NYNU 2211328 was isolated from a leaf of Akebia trifoliata collected from the Baotianman Nature Reserve in Henan Province central China. Phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region suggested that this strain is closely related to Naohidea sebacea CBS 8477 exhibiting the similarity values of 96.5% and 91.3% in the D1/D2 domain and the ITS region respectively. Physiologically the novel strain differs from N. sebacea in its ability to assimilate inulin trehalose and d-arabinose its inability to assimilate dl-lactate and its incapability of growth at 30 °C. Both phenotypic and phylogenetic analyses indicated that the isolated strain represents a novel species in the genus Naohidea and the name Naohidea akebiae fa. sp. nov. (holotype: CICC 33584; MycoBank number: MB 855585) is proposed.
Kondoa tianchiensis f.a., sp. nov., an anamorphic yeast species isolated from plant leaves
Two yeast strains NYNU 236122 and NYNU 236180 were isolated from plant leaves collected in Tianchi Mountain Henan Province central China. Molecular phylogenetic analyses revealed the closest relatives of the strains are three described Kondoa species Kondoa chamaenerii Kondoa miscanthi and Kondoa subrosea. Genetically the isolated strains differed from the type strains of their three related species by 2–11(0.2–1.8%) base substitutions in the D1/D2 domain 16–40 (2.6–5.6%) base mismatches in the internal transcribed spacer region and more than 10.1% base substitutions in the partial RPB2 gene. Furthermore the two strains differ physiologically from their closest related species K. chamaenerii in their ability to assimilate dl-lactate nitrite and l-lysine and their inability to assimilate nitrate. Additionally they differ from K. miscanthi and K. subrosea in their ability to assimilate inulin d-gluconate and l-lysine. The species name of Kondoa tianchiensis f.a. sp. nov. is proposed with holotype CICC 33616T (Mycobank MB 853544).
Cyberlindnera qingyuanensis f.a., sp. nov., a yeast species isolated from rotting wood
Two yeast strains were isolated from rotting wood samples collected on Qingyuan Mountain Fujian Province PR China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit rRNA gene revealed that these two strains represent a novel species of the genus Cyberlindnera. The proposed name for this new species is Cyberlindnera qingyuanensis f.a. sp. nov. (holotype: GDMCC 2.300; ex-type: PYCC 9925) although the formation of ascospores was not observed. The novel species differs from its close relative Cyberlindnera galapagoensis by 7.7% sequence divergence (37 substitutions and seven indels) in the D1/D2 domain and 9.7% sequence divergence (42 substitutions and 34 indels) in the ITS region respectively. Additionally Cyb. qingyuanensis differs from its close relative Cyb. galapagoensis by its ability to grow in cellobiose l-rhamnose ribitol galactitol and dl-lactate its growth at 37 °C and its inability to ferment raffinose. The Mycobank number is MB 854693.
Pichia kurtzmaniana f.a. sp. nov., with the transfer of eight Candida species to Pichia
Three yeast strains belonging to the ascomycetous yeast genus Pichia were isolated from two soil samples from Yunnan and Guizhou provinces and a marine water sample from Liaoning province PR China. Phylogenetic analyses based on the sequences of the D1/D2 domains of the large subunit(LSU) rRNA gene and the internal transcribed spacer (ITS) region indicate that these three strains together with 12 additional strains isolated from various substrates collected in different regions or countries of the world represent a novel species of the genus Pichia for which the name Pichia kurtzmaniana sp. nov. (holotype: strain CGMCC 2.7213) is proposed. The novel species differs from its close relatives Candida californica by eight (1.5 %) and 26 (11.1 %) mismatches in the D1/D2 domains and the ITS region respectively; and from Pichia chibodasensis by 11 (2.1 %) and 20 (8.7 %) mismatches in the D1/D2 domains and the ITS region respectively. In addition eight Candida species which belong to the Pichia clade are transferred to the genus Pichia resulting in the proposal of the following new combinations: Pichia cabralensis comb. nov. Pichia californica comb. nov. Pichia ethanolica comb. nov. Pichia inconspicua comb. nov. Pichia phayaonensis comb. nov. Pichia pseudolambica comb. nov. Pichia rugopelliculosa comb. nov. and Pichia thaimueangensis comb. nov.
Saturnispora sinensis sp. nov., a new ascomycetous yeast species from soil and rotten wood
A yeast strain (CGMCC 2.6937T) belonging to the ascomycetous yeast genus Saturnispora was recently isolated from soil collected in Xinghuacun Shanxi Province PR China. The strain produces one or two ellipsoid or spherical ascospores in asci formed by the conjugation between a cell and its bud. Phylogenetic analyses of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit rRNA gene suggest that this strain is conspecific with strains NYNU 14639 isolated from rotten wood collected in Funiu Mountain Henan province and ES13S05 from soil collected in Nantou County Taiwan. The CGMCC 2.6937T group is most closely related to Saturnispora dispora and Saturnispora zaruensis. However strain CGMCC 2.6937T differs from S. dispora by 17 (3.2 % 13 substitutions and four gaps) and 77 (18.8 % 52 substitutions and 25 gaps) mismatches and from S. zaruensis by 15 (2.9 % 12 substitutions and three gaps) and 64 (15.6 % 44 substitutions and 20 gaps) mismatches in the D1/D2 domain and ITS region respectively. The results suggest that the CGMCC 2.6937T group represents an undescribed species in the genus Saturnispora for which the name Saturnispora sinensis sp. nov. is proposed. The holotype strain is CGMCC 2.6937T.
Spencermartinsiella henanensis fa., sp. nov., a novel yeast species isolated from rotting wood
Two yeast strains (NYNU 211162 and NYNU 211275) were isolated from rotting wood collected in the Baotianman Nature Reserve Henan Province central China. Phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that the strains represent a phylogenetically distinct species within the genus Spencermartinsiella. The name Spencermartinsiella henanensis fa. sp. nov. is proposed for this species with holotype CICC 33543T (Mycobank MB 851142). S. henanensis sp. nov. differed by only 3 nt (~0.5 %) substitutions from the closest known species S. europaea NCAIM Y.01817T in the D1/D2 domain but by 33 nt (~6 %) substitutions 34 nt (~3.8 %) substitutions 30 nt (~5.6 %) substitutions and 75 nt (~9.9 %) substitutions in the ITS region and the partial TEF1 COXII and RPB2 genes. Additionally S. henanensis sp. nov. can be physiologically distinguished from S. europaea by its ability to assimilate inulin inability to assimilate ethylamine and cadaverine and incapability of growth at 30 °C.
Danielozyma pruni sp. nov., an asexual yeast species isolated from insect frass
Two strains (NYNU 218101 and NYNU 218104) of an asexual yeast species were isolated from insect frass collected in insect tunnels of red leaf plum trees in the Henan Province central China. Molecular phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region showed that these two strains belonged to the genus Danielozyma with Danielozyma litseae as the closest known species. They differed from the type strain of D. litseae by 0.6 % substitutions (three substitutions and one gap) in the D1/D2 domain and by 5.1 % substitutions (19 substitutions and six gaps) in the ITS region respectively. When compared with the partial ACT1 TEF1 and RPB1 gene sequences they differed by 3 % (26 substitutions) 2.7 % (25 substitutions) and 9 %(54 substitutions) from D. litseae NRRL YB-3246T in these regions. Physiologically they also differed from its closest known species D. litseae based on the ability to assimilate inulin and galactitol as well as to grow in 0.1 % cycloheximide and its inability to ferment maltose and raffinose. In order to classify the two new isolates based on morphological and molecular evidence we proposed the description of a novel species Danielozyma pruni sp. nov. with strain JCM 35735T as holotype (Mycobank MB 849101).
Hanseniaspora menglaensis f.a., sp. nov., a novel apiculate yeast species isolated from rotting wood
Two apiculate strains (NYNU 181072 and NYNU 181083) of a bipolar budding yeast species were isolated from rotting wood samples collected in Xishuangbanna Tropical Rainforest in Yunnan Province southwest PR China. On the basis of phenotypic characteristics and the results of phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA internal transcribed spacer (ITS) region and the actin (ACT1) gene the two strains were found to represent a single novel species of the genus Hanseniaspora for which the name Hanseniaspora menglaensis f.a. sp. nov. (holotype CICC 33364T; MycoBank MB 847437) is proposed. In the phylogenetic tree H. menglaensis sp. nov. showed a close relationship with Hanseniaspora lindneri Hanseniaspora mollemarum Hanseniaspora smithiae and Hanseniaspora valbyensis. H. menglaensis sp. nov. differed from H. lindneri the most closely related known species by 1.2 % substitutions in the D1/D2 domain 2.5 % substitutions in the ITS region and 5.4 % substitutions in the ACT1 gene respectively. Physiologically H. menglaensis sp. nov. can also be distinguished from H. lindneri by its ability to assimilate d-gluconate.
Spencerozyma pingqiaoensis sp. nov., a yeast species isolated from the external surface of rice leaves in China
Four strains (NYNU 178247 NYNU 178251 DMKU-PAL160 and DMKU-PAL137) representing a novel yeast species were isolated from the external surfaces of rice and pineapple leaves collected in China and Thailand. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit rRNA gene revealed that the novel species belonged to the genus Spencerozyma. The D1/D2 sequence of the novel species differed from its closest relative Spencerozyma acididurans SYSU-17T by 3.2 % sequence divergence. The species also differed from Spencerozyma crocea CBS 2029T and Spencerozyma siamensis DMKU13-2T by 3.0–6.9 % sequence divergence in the D1/D2 sequences out of 592 bp. In the ITS regions the novel species displayed 19.8–29.2% sequence divergence from S. acididurans SYSU-17T S. crocea CBS 2029T and S. siamensis DMKU13-2T out of 655 bp. Furthermore the novel species could also be differentiated from the closely related species by some physiological characteristics. The species name of Spencerozyma pingqiaoensis sp. nov. (Holotype CBS 15238 Mycobank MB 844734) is proposed to accommodate these four strains.
Barnettozyma menglunensis f.a., sp. nov., a novel yeast species isolated from rotting wood
A novel yeast species is described based on three strains isolated from rotting wood samples from Xishuangbanna Tropical Rainforest in Yunnan Province PR China. Strain NYNU 1811121 was isolated in Menglun Mengla while strains NYNU 18982 and NYNU 181096 were recovered in Mengyang Jinghong. Analysis of the sequences of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) revealed that the novel strains were closely related to the type strain of [Candida] sanyiensis but with 6.9 % nucleotide substitutions in the D1/D2 domain and 8.2 % substitutions in the ITS region. The three novel strains can also be distinguished from C. sanyiensis in terms of the ability to assimilate trehalose and d-gluconate and to grow at 35 °C as well as the inability to ferment glucose. Based on molecular analyses and phenotypic characteristics the name Barnettozyma menglunensis f.a. sp. nov. is proposed with the holotype CBS 16011T (MycoBank 845375).
Torulaspora jiuxiensis sp. nov., a novel yeast species isolated from rotting wood
Two strains of a novel ascomycetous yeast species were isolated from rotting wood samples collected in Jiuxi Mountain Forest Park in Yunnan Province southwest China. Both strains formed one or two spherical ascospores in persistent asci. Phylogenetic analysis of the concatenated sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and the D1/D2 domain of the large subunit rRNA gene revealed that the novel strains represented a phylogenetically distinct species belonging to the genus Torulaspora. This novel species differed from the type strains of the closest known species Torulaspora nypae and Torulaspora maleeae by 0.9 and 1.2 % nucleotide substitutions in the D1/D2 domain and 5.3 and 6 % nucleotide substitutions in the ITS region respectively. The novel species can also be distinguished from T. nypae and T. maleeae in terms of the ability to assimilate ribitol succinate and citrate and its ability to grow at 37 °C. The species name of Torulaspora jiuxiensis sp. nov. is proposed with holotype CBS 16004T (Mycobank MB 844535).
Hyphopichia xiaguanensis f.a., sp. nov., an ascomycetous yeast species isolated from plant leaves
Two strains representing a novel yeast species were isolated from plant leaves collected in the Baotianman Nature Reserve in Henan Province central China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and the D1/D2 domain of the large subunit rRNA gene revealed that the novel species belonged to the genus Hyphopichia although the formation of ascospores was not observed. The novel species was related most closely to Hyphopichia paragotoi CBS 13913T but they differed by 0.9 % sequence divergence (five substitutions) in the D1/D2 domain and by 3.7 % sequence divergence (seven substitutions and eight gaps) in the ITS region. Furthermore the novel species can also be differentiated from the closely related species in some biochemical and physiological characteristics. The species name of Hyphopichia xiaguanensis f.a. sp. nov. (Holotype CBS 16668 Mycobank MB 842425) is proposed to accommodate strains NYNU 20899T and NYNU 20914.
Blastobotrys baotianmanensis sp. nov. and Blastobotrys xishuangbannaensis f.a., sp. nov., two novel yeast species associated with insects and rotting wood
Five yeast strains were isolated from the gut of the groundbeetle Pterostichus gebleri and rotting wood which were collected from two different localities in China. These strains were identified as representing two novel species of the genus Blastobotrys through comparison of sequences in the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. Blastobotrys baotianmanensis sp. nov. produces two to three spherical ascospores per ascus and is most closely related to the type strains of B. elegans B. capitulata B. arbuscula and an undescribed species represented by strain BG02-7-20-006A-3-1. Blastobotrys baotianmanensis sp. nov. differed from these strains by 3.6–8.4 % divergence (21–46 substitutions and 0–4 gaps) in the D1/D2 sequences. Blastobotrys xishuangbannaensis f.a. sp. nov. is closely related to B. nivea B. elegans and B. aristata but the formation of ascospores was not observed on various sporulation media and it differed from its relatives by 6.2–8.5 % divergence (34–43 substitutions and 2–6 gaps) in the D1/D2 sequences. The holotype of Blastobotrys baotianmanensis sp. nov. is NYNU 1581 and the holotype of Blastobotrys xishuangbannaensis f.a. sp. nov. is NYNU 181030.
Kazachstania jinghongensis sp. nov. and Kazachstania menglunensis f.a., sp. nov., two yeast species isolated from rotting wood
Five yeast strains were isolated from rotting wood samples collected in the Xishuangbanna Tropical Rainforest Yunnan Province PR China. Phylogenetic analysis of the D1/D2 domains of the large subunit rRNA gene indicated that these strains represent two novel species of the genus Kazachstania. Kazachstania jinghongensis sp. nov. produces one to two spherical ascospores per ascus and is most closely related to Kazachstania lodderae and Kazachstania spencerorum. Kazachstania jinghongensis sp. nov. differed from the type strains of the two latter species by 13–24 substitutions in the D1/D2 domains and by 39–56 substitutions in the ITS regions. Kazachstania menglunensis f.a. sp. nov. is a member of the Kazachstania jiainica subclade but the formation of ascospores was not observed on various sporulation media. Kazachstania menglunensis sp. nov. differed from other members of the subclade by 23–26 substitutions in the D1/D2 domains and by more than 67 substitutions in the ITS regions. The holotype of Kazachstania jinghongensis sp. nov. is NYNU 17944 (CBS 15232) and the holotype of Kazachstania menglunensis sp. nov. is NYNU 18913 (CBS 16054).
Metschnikowia baotianmanensis f.a., sp. nov., a new yeast species isolated from the gut of the rhinoceros beetle Allomyrina dichotoma
Four strains NYNU 15610 NYNU 15612 NYNU 15613 and NYNU 15615 of a novel ascomycetous yeast were isolated from the gut of Allomyrina dichotoma (Coleoptera: Scarabeidae) collected from two different localities in Henan Province Central PR China. The four strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions. Sequence analyses revealed that this novel species represents a member of the genus Metschnikowia. It differed from its closest known species Metschnikowia zobellii Metschnikowiaaustralis and Metschnikowia bicuspidata by 8.4–9.2 % sequence divergence (33–40 nt substitutions and 7–12 gaps over 509 bases) in the D1/D2 sequences. The formation of ascospores was not observed on various sporulation media. In contrast to M. zobellii M. australis and M. bicuspidata the novel yeast species was unable to assimilate succinate ethanol ethylamine cadaverine and 10 % NaCl plus 5 % glucose but was able to grow in vitamin-free medium. The name Metschnikowia baotianmanensis f.a. sp. nov. is proposed to accommodate these strains with NYNU 15613 as the holotype.
Candida yunnanensis sp. nov. and Candida parablackwelliae sp. nov., two yeast species in the Candida albicans/Lodderomyces clade
During studies on the yeast communities associated with rotting wood in the Xishuangbanna Tropical Rainforest in PR China four novel yeast strains were found. Phylogenetic analysis based on the concatenated sequences of the D1/D2 domains of the large subunit rRNA gene and the ITS regions showed that these strains represented two novel species in the Candida albicans/Lodderomyces clade. The novel species represented by strains NYNU 17948 and NYNU 17981 formed a clade with Candida maltosa and Candida baotianmanensis with 1–1.8% sequence divergence in the D1/D2 domains and 8.9–10% sequence divergence in the ITS regions. The other novel species represented by NYNU 17105 and NYNU 17763 is most closely related to Candida blackwelliae with 0.7 % sequence divergence in the D1/D2 domains and 6.9 % sequence divergence in the ITS regions. The two novel species could be distinguished from their closest described species in terms of physiological traits. The two novel species are described as Candida yunnanensis sp. nov. (holotype NYNU 17948) and Candida parablackwelliae sp. nov. (holotype NYNU 17763).
Saturnispora galanensis sp. nov., a yeast species isolated from rotting wood
Three strains of a novel yeast species were isolated from rotting wood in the Xishuangbanna Tropical Rainforest Yunnan Province PR China. Sequence analysis of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions showed that the novel species represents a member of the genus Saturnispora. It differed from its closest known species Saturnispora sekii CBS 10931T by 1.3 % nucleotide substitutions in the D1/D2 domains and by 2.2 % nucleotide substitutions in the ITS regions respectively. In contrast to Saturnispora sekii the novel yeast species was unable to assimilate glycerol dl-lactate succinate and citrate and grow at 37 °C. The name Saturnispora galanensis sp. nov. is proposed to accommodate these strains with NYNU 1797 as the holotype.
Mucor chuxiongensis sp. nov., a novel fungal species isolated from rotten wood
Three strains of a novel mucoralean fungus were isolated from samples of decayed wood which were collected from three locations near the city of Chuxiong Yunnan province China. These isolates were identified as a novel species through comparison of sequences in the ITS sequence the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. The results demonstrated that these isolates represent a novel mucoralean fungus species belonging to the genus Mucor. The ITS sequence of Mucor chuxiongensissp. nov. differed from its closest relative Mucor guilliermondii CBS 174.27T by 13.1 % sequence divergence (39 substitutions and 38 gaps) and the D1/D2 sequences of the novel strains differed by 13 nt substitutions and one gap (1.9 %) from the ex-type strain. On potato dextrose agar malt extract agar and synthetic mucor agar the isolates grew slowly below 10 °C rapidly at 25–30 °C and could not grow well at 35 °C. The holotype strain of Mucor chuxiongensis sp. nov. is NYNU 174111 (CICC 41666T=CBS 143707T).