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Abstract

Two novel yeast strains, NYNU 236247 and NYNU 23523, were isolated from the leaves of Hance, collected in the Tianchi Mountain National Forest Park, Henan Province, central China. Phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region revealed the closest relatives of the strains are three described species: , and . The novel species differed from the type strains of these three species by 12 to 22 nucleotide substitutions and 1 gap (~2.0–4.0%) in the D1/D2 domain and by 78 to 100 nucleotide mismatches (~12.0–16%) in the ITS region. Physiologically, the novel species differs from and in its ability to assimilate -lactate and melezitose and from by its inability to assimilate melibiose, soluble starch and ethanol. sp. nov. is proposed for those two strains, with the holotype designated as GDMCC 2.483 (MycoBank MB 857072).

Funding
This study was supported by the:
  • Open-ended project of Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, P. R. China (Award 2021ZTSJJ5)
    • Principle Award Recipient: Chun-YueChai
  • Key Scientific Research Project of Colleges and Universities in Henan Province (Award 23A210018)
    • Principle Award Recipient: Chun-YueChai
  • State Key Laboratory for Management and Control of Complex Systems (Award MIMCP-202403)
    • Principle Award Recipient: Chun-YueChai
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2025-01-13
2025-01-14
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