RESULTS:
1 - 8 of 8 for "Chun-Yue Chai"
Pseudobensingtonia carpini sp. nov., a novel yeast species isolated from plant leaves in China
Two novel yeast strains NYNU 236247 and NYNU 23523 were isolated from the leaves of Carpinus turczaninowii Hance collected in the Tianchi Mountain National Forest Park Henan Province central China. Phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region revealed the closest relatives of the strains are three described Pseudobensingtonia species: Ps. fusiformis Ps. musae and Ps. ingoldii. The novel species differed from the type strains of these three species by 12 to 22 nucleotide substitutions and 1 gap (~2.0–4.0%) in the D1/D2 domain and by 78 to 100 nucleotide mismatches (~12.0–16%) in the ITS region. Physiologically the novel species differs from Ps. fusiformis and Ps. musae in its ability to assimilate dl-lactate and melezitose and from Ps. ingoldii by its inability to assimilate melibiose soluble starch and ethanol. Pseudobensingtonia carpini sp. nov. is proposed for those two strains with the holotype designated as GDMCC 2.483T (MycoBank MB 857072).
Spencerozyma pingqiaoensis sp. nov., a yeast species isolated from the external surface of rice leaves in China
Four strains (NYNU 178247 NYNU 178251 DMKU-PAL160 and DMKU-PAL137) representing a novel yeast species were isolated from the external surfaces of rice and pineapple leaves collected in China and Thailand. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit rRNA gene revealed that the novel species belonged to the genus Spencerozyma. The D1/D2 sequence of the novel species differed from its closest relative Spencerozyma acididurans SYSU-17T by 3.2 % sequence divergence. The species also differed from Spencerozyma crocea CBS 2029T and Spencerozyma siamensis DMKU13-2T by 3.0–6.9 % sequence divergence in the D1/D2 sequences out of 592 bp. In the ITS regions the novel species displayed 19.8–29.2% sequence divergence from S. acididurans SYSU-17T S. crocea CBS 2029T and S. siamensis DMKU13-2T out of 655 bp. Furthermore the novel species could also be differentiated from the closely related species by some physiological characteristics. The species name of Spencerozyma pingqiaoensis sp. nov. (Holotype CBS 15238 Mycobank MB 844734) is proposed to accommodate these four strains.
Blastobotrys baotianmanensis sp. nov. and Blastobotrys xishuangbannaensis f.a., sp. nov., two novel yeast species associated with insects and rotting wood
Five yeast strains were isolated from the gut of the groundbeetle Pterostichus gebleri and rotting wood which were collected from two different localities in China. These strains were identified as representing two novel species of the genus Blastobotrys through comparison of sequences in the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. Blastobotrys baotianmanensis sp. nov. produces two to three spherical ascospores per ascus and is most closely related to the type strains of B. elegans B. capitulata B. arbuscula and an undescribed species represented by strain BG02-7-20-006A-3-1. Blastobotrys baotianmanensis sp. nov. differed from these strains by 3.6–8.4 % divergence (21–46 substitutions and 0–4 gaps) in the D1/D2 sequences. Blastobotrys xishuangbannaensis f.a. sp. nov. is closely related to B. nivea B. elegans and B. aristata but the formation of ascospores was not observed on various sporulation media and it differed from its relatives by 6.2–8.5 % divergence (34–43 substitutions and 2–6 gaps) in the D1/D2 sequences. The holotype of Blastobotrys baotianmanensis sp. nov. is NYNU 1581 and the holotype of Blastobotrys xishuangbannaensis f.a. sp. nov. is NYNU 181030.
Metschnikowia baotianmanensis f.a., sp. nov., a new yeast species isolated from the gut of the rhinoceros beetle Allomyrina dichotoma
Four strains NYNU 15610 NYNU 15612 NYNU 15613 and NYNU 15615 of a novel ascomycetous yeast were isolated from the gut of Allomyrina dichotoma (Coleoptera: Scarabeidae) collected from two different localities in Henan Province Central PR China. The four strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions. Sequence analyses revealed that this novel species represents a member of the genus Metschnikowia. It differed from its closest known species Metschnikowia zobellii Metschnikowiaaustralis and Metschnikowia bicuspidata by 8.4–9.2 % sequence divergence (33–40 nt substitutions and 7–12 gaps over 509 bases) in the D1/D2 sequences. The formation of ascospores was not observed on various sporulation media. In contrast to M. zobellii M. australis and M. bicuspidata the novel yeast species was unable to assimilate succinate ethanol ethylamine cadaverine and 10 % NaCl plus 5 % glucose but was able to grow in vitamin-free medium. The name Metschnikowia baotianmanensis f.a. sp. nov. is proposed to accommodate these strains with NYNU 15613 as the holotype.
Candida yunnanensis sp. nov. and Candida parablackwelliae sp. nov., two yeast species in the Candida albicans/Lodderomyces clade
During studies on the yeast communities associated with rotting wood in the Xishuangbanna Tropical Rainforest in PR China four novel yeast strains were found. Phylogenetic analysis based on the concatenated sequences of the D1/D2 domains of the large subunit rRNA gene and the ITS regions showed that these strains represented two novel species in the Candida albicans/Lodderomyces clade. The novel species represented by strains NYNU 17948 and NYNU 17981 formed a clade with Candida maltosa and Candida baotianmanensis with 1–1.8% sequence divergence in the D1/D2 domains and 8.9–10% sequence divergence in the ITS regions. The other novel species represented by NYNU 17105 and NYNU 17763 is most closely related to Candida blackwelliae with 0.7 % sequence divergence in the D1/D2 domains and 6.9 % sequence divergence in the ITS regions. The two novel species could be distinguished from their closest described species in terms of physiological traits. The two novel species are described as Candida yunnanensis sp. nov. (holotype NYNU 17948) and Candida parablackwelliae sp. nov. (holotype NYNU 17763).
Saturnispora galanensis sp. nov., a yeast species isolated from rotting wood
Three strains of a novel yeast species were isolated from rotting wood in the Xishuangbanna Tropical Rainforest Yunnan Province PR China. Sequence analysis of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions showed that the novel species represents a member of the genus Saturnispora. It differed from its closest known species Saturnispora sekii CBS 10931T by 1.3 % nucleotide substitutions in the D1/D2 domains and by 2.2 % nucleotide substitutions in the ITS regions respectively. In contrast to Saturnispora sekii the novel yeast species was unable to assimilate glycerol dl-lactate succinate and citrate and grow at 37 °C. The name Saturnispora galanensis sp. nov. is proposed to accommodate these strains with NYNU 1797 as the holotype.
Mucor chuxiongensis sp. nov., a novel fungal species isolated from rotten wood
Three strains of a novel mucoralean fungus were isolated from samples of decayed wood which were collected from three locations near the city of Chuxiong Yunnan province China. These isolates were identified as a novel species through comparison of sequences in the ITS sequence the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. The results demonstrated that these isolates represent a novel mucoralean fungus species belonging to the genus Mucor. The ITS sequence of Mucor chuxiongensissp. nov. differed from its closest relative Mucor guilliermondii CBS 174.27T by 13.1 % sequence divergence (39 substitutions and 38 gaps) and the D1/D2 sequences of the novel strains differed by 13 nt substitutions and one gap (1.9 %) from the ex-type strain. On potato dextrose agar malt extract agar and synthetic mucor agar the isolates grew slowly below 10 °C rapidly at 25–30 °C and could not grow well at 35 °C. The holotype strain of Mucor chuxiongensis sp. nov. is NYNU 174111 (CICC 41666T=CBS 143707T).
Wickerhamomyces menglaensis f.a., sp. nov., a yeast species isolated from rotten wood
Five strains NUNU 16637 NYNU 16645 NYNU 1673 NYNU 1680 and NYNU 1689 of a novel ascomycetous yeast were isolated from the Xishuangbanna tropical rainforest Yunnan Province PR China. The five strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions. Sequence analysis showed that they represent undescribed yeast species belonging to the genus Wickerhamomyces. They differed from their closest known species Wickerhamomyces xylosivorus NBRC 111553T by 3.4 % sequence divergence (14 substitutions and six gaps out of 584 bp) in the D1/D2 domains and by 9.6 % sequence divergence (28 substitutions and 24 gaps over 543 bp) in the ITS regions respectively. The five strains of novel species reproduced asexually; no sexual reproduction could be found. In contrast to W. xylosivorus the novel yeast species were able to assimilate l-arabinose inulin soluble starch d-mannitol and citrate and unable to assimilate trehalose raffinose 5-keto-d-gluconate d-gluconate ethanol ethylamine and cadaverine. Growth was observed at 35 °C. The name Wickerhamomyces menglaensis f.a. sp. nov. is proposed to accommodate these strains with NYNU 1673 as the holotype.