RESULTS:
1 - 3 of 3 for "Mikako Ito"
Desulfovibrio falkowii sp. nov., Porphyromonas miyakawae sp. nov., Mediterraneibacter flintii sp. nov. and Owariibacterium komagatae gen. nov., sp. nov., isolated from human faeces
Small obligately anaerobic strains 13CB8CT 13CB11CT 13CB18CT and 13GAM1GT were isolated from a faecal sample in a patient with Parkinson’s disease with a history of duodenal resection. After conducting a comprehensive polyphasic taxonomic analysis including genomic analysis we propose the establishment of one new genus and four new species. The novel bacteria are Desulfovibrio falkowii sp. nov. (type strain JCM 36128T = DSM 116810T) Porphyromonas miyakawae sp. nov. (type strain JCM 36129T = DSM 116947T) Mediterraneibacter flintii sp. nov. (type strain JCM 36130T = DSM 116866T) and Owariibacterium komagatae gen. nov. sp. nov. (type strain JCM 36131T = DSM 116982T) respectively.
Common marmoset (Callithrix jacchus) as a primate model of dengue virus infection: development of high levels of viraemia and demonstration of protective immunity
Dengue virus (DENV) causes a wide range of illnesses in humans: dengue fever (DF) dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Animal models that constantly develop high levels of viraemia are required for the development of protective and preventive measures. Common marmosets (Callithrix jacchus) demonstrated high levels of viraemia after inoculation with clinical isolates of four serotypes of DENV; in particular over 106 genome copies ml−1 after inoculation with DENV-2. Non-structural protein 1 and DENV-specific IgM and IgG antibodies were consistently detected. The DENV-2 genome was detected in lymphoid organs including the lymph nodes spleen and thymus and also in non-lymphoid organs. DENV antigen was detected by immunohistochemistry in the liver and spleen from inoculated marmosets. Four marmosets were reinoculated with DENV-2 at 33 weeks after primary inoculation with DENV-2. The DENV-2 genome was not detected in any of these marmosets indicating protection from a secondary infection. The results indicate that common marmosets are highly sensitive to DENV infection and suggest that marmosets could be a reliable primate model for the evaluation of candidate vaccines.
Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004
To characterize Japanese encephalitis virus (JEV) strains recently prevalent in Japan JEV surveillance was performed in pigs from 2002 to 2004. Eleven new JEV isolates were obtained and compared with previous isolates from Japan and other Asian countries. All of the isolates were classified into genotype 1 by nucleotide sequence analysis of the E gene. Two new isolates with different levels of neurovirulence and neuroinvasiveness but with only one nucleotide difference in the E gene Sw/Mie/34/2004 and Sw/Mie/40/2004 were isolated at the same farm on the same day. Sw/Mie/40/2004 displayed higher neurovirulence and neuroinvasiveness in mice than the other four new isolates. Another new isolate Sw/Hiroshima/25/2002 was neutralized by antiserum to Beijing-1 at a level similar to the homologous Beijing-1 strain whilst seven other new isolates were neutralized at 10-fold-lower titres. However there were no amino acid differences in the E protein among these eight isolates. The present study indicated that the 11 new JEV isolates were genetically similar but biologically and serologically heterogeneous.