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Volume 6, Issue 7

Phenotypic and genotypic characterization of extended-spectrum beta-lactamase-producing in a Moroccan hospital Open Access

Abstract

Extended-spectrum beta-lactamase-producing (ESBL-E) is a major public health problem in hospitals and in the community. The objective of this work was to describe the epidemiology of ESBL-E to study their resistance profile and to determine the genes encoding the ESBL phenotype. This is a retrospective study conducted in the bacteriology laboratory of the Mohamed V Military Training Hospital in Rabat, and covering all isolates of from 1 January 2018 to 31 December 2020. The molecular study of ESBL genes involved a representative sample of all ESBL isolates. The overall prevalence of ESBLs in isolated (1402/10268) is 13.65 %. The urinary tract was the main site of isolation of ESBL (61 %). The bacterial species most concerned are (41.9 %), (42.2 %) and (11.9 %). The study of antibiotic susceptibility showed a resistant profile marked mainly by 100 % resistance to first generation cephalosporins (1GC) and third generation cephalosporins (3GC), 55 % to piperacillin-tazobactam, 16 % to imipenem, and 87 % to fluoroquinolones. Molecular typing of ESBL strains showed a prevalence of CTX-M (95 %), SHV (50 %) and TEM (56 %). The CTX-M-1 and the CTX-M-9 groups were the most common (96.19 % and 7.62 % respectively), and CTX-M15 was found in 78.10 % of CTX-M-1 ESBL positive isolates. Most strains had more than two coexisting resistance genes. The prevalence rate of ESBL-E is critical, and preventive action at different levels (prescriber, biologist, hospital, patient, etc.) are necessary in order to limit their spread and to manage a better therapeutic strategy.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial resistance , ESBL , multi-drug resistant bacteria , phenotypic and genotypic characterization and prevalence
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
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2024-07-17
2025-01-14
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/content/journal/acmi/10.1099/acmi.0.000822.v3
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Phenotypic and genotypic characterization of extended-spectrum beta-lactamase-producing Enterobacteriaceae in a Moroccan hospital
Access Microbiology 6, 000822.v3 (2024); https://doi.org/10.1099/acmi.0.000822.v3
/content/journal/acmi/10.1099/acmi.0.000822.v3
/content/journal/acmi/10.1099/acmi.0.000822.v3
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