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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 4

sp. nov., isolated from soil samples of an organic paddy in Japan No Access

Abstract

Two Gram-stain-negative, terminal endospore-forming, rod-shaped and aerotolerant bacterial strains designated D1-1 and B3 were isolated from soil samples of an organic paddy in Japan. Strain D1-1 grew at 15–37 °C, pH 5.0–7.3, and with up to 0.5 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene revealed that strain D1-1 belonged to the genus and was closely related to CSC2 (99.7 % sequence similarity), TW1 (99.7 %) and CT4 (99.3 %). Strains D1-1 and B3 were whole-genome sequenced and indistinguishable, with an average nucleotide identity value of 99.7 %. The average nucleotide identity (below 91.1 %) and digital DNA–DNA hybridization (below 43.6 %) values between the two novel isolates and their corresponding relatives showed that strains D1-1 and B3 could be readily distinguished from their closely related species. A novel species, sp. nov., with type strain D1-1 (=MAFF 212477=DSM 113523), is proposed based on genotypic and phenotypic data.

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  • Accepted:
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Keyword(s): Bacillota , Clostridium folliculivorans sp.nov and paddy
Funding
This study was supported by the:
  • NARO NIP project (Award 2022)
    • Principle Award Recipient: JunkoTazawa
  • the Genebank project (Microorganism Section) (Award 2022)
    • Principle Award Recipient: MasanoriTohno
  • JSPS KAKENHI (Award JP21K05552)
    • Principle Award Recipient: JunkoTazawa
  • JSPS KAKENHI (Award JP21K14967)
    • Principle Award Recipient: HisamiKobayashi
© 2023 The Authors
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2023-04-27
2025-01-14
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/content/journal/ijsem/10.1099/ijsem.0.005876
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Clostridium folliculivorans sp. nov., isolated from soil samples of an organic paddy in Japan
Int J Syst Evol Microbiol 73, 005876 (2023); https://doi.org/10.1099/ijsem.0.005876
/content/journal/ijsem/10.1099/ijsem.0.005876
/content/journal/ijsem/10.1099/ijsem.0.005876
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