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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 73, Issue 10

Genomic characterization of harbouring an exogenous resistance gene No Access

Abstract

Reports of β-lactamase-producing are increasing worldwide.

. This study aimed to elucidate the molecular characteristics and evolution of β-lactamase-producing .

A total of 159 clinical isolates were characterized using multi-locus sequence typing. Antimicrobial resistance genes and integrative and conjugative element (ICE) types were identified through PCR and DNA sequencing. The genetic structure of ICE was further investigated using whole-genome sequencing.

Out of 159 clinical isolates, 20.8% (=33) were β-lactamase producers. Thirteen sequence types (STs) were identified. ST 103, 155, 165 and 388 have been identified in previous studies, suggesting that strains with these STs tend to acquire the β-lactamase gene . Among β-lactamase producers, 66.7% (=22) of were located on ICE. The ICEs could be classified into two groups based on their sequence (types I and II). Among these strains, 2017-Y3 harboured a macrolide resistance gene, , in ICE. A comparative analysis of the ICE region of this strain and those from other countries suggested that each isolate was derived from ICE type I or II. These regions, including ), were similar to those of Tn, which is commonly found in .

This study revealed several STs associated with the acquisition of β-lactamase genes on ICEs. Additionally, ICE evolution involved the acquisition of exogenous genes. The accumulation of resistance genes in ICE raises concerns regarding the emergence of multidrug-resistant .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): exogenous resistance gene , Haemophilus influenzae , integrative and conjugative element and β-lactamase
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 20H01077)
    • Principle Award Recipient: ShojiSeyama
  • Research Institute of Meijo University
    • Principle Award Recipient: TakeakiWajima
  • Takeda Science Foundation
    • Principle Award Recipient: TakeakiWajima
  • Japan Society for the Promotion of Science (Award 23KJ1955)
    • Principle Award Recipient: EmiTanaka
© 2024 The Authors
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2024-10-03
2025-01-14
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Genomic characterization of Haemophilus influenzae harbouring an exogenous resistance gene
J. Med. Microbiol. 73, 001904 (2024); https://doi.org/10.1099/jmm.0.001904
/content/journal/jmm/10.1099/jmm.0.001904
/content/journal/jmm/10.1099/jmm.0.001904
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