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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov., isolated from the intestines of No Access

Abstract

A Gram-stain-negative, aerobic, motile, catalase-positive, oxidase-positive, short rod-shaped marine bacterium, designated as YIC-827, was isolated from Qingdao, Shandong Province, China. The results showed that cells of strain YIC-827 could grow optimally at 25–35 °C, pH 6.5–7.5 and 2–7% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that the strain YIC-827 was a member of the genus . The closest relative to this strain was KMM 300, with a similarity of 98.39%. The digital DNA–DNA hybridization value between the new isolate and phylogenetically related species is 19.6%. Strain YIC-827 could decompose sodium alginate, casein and esters (Tween 20, Tween 40, Tween 60 and Tween 80), but could not hydrolyse starch, cellulose and DNA. The fatty acid profile of a strain consists of a large number of C, C ω7c and C ω7c/ ω6c. The G+C content of the DNA of this strain was determined to be 48.93%. Based on phenotypic characteristics, phylogenetic analysis and DNA–DNA correlation data, the strain YIC-827 represents a novel species of the genus with the name sp. nov. The type strain of sp. is strain YIC-827 (=MCCC 1K08807=CGMCC 1.62085=KCTC 8212).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , DNA–DNA relatedness , polyphasic taxonomy and Pseudoalteromonas qingdaonensis sp. nov.
Funding
This study was supported by the:
  • Liaoning Provincial Undergraduate Colleges and Universities Basic Research Funds (Award LJ202410166008)
    • Principle Award Recipient: MaLianju
  • the Research Fund for the Taishan Scholar Project of Shandong Province of China (Award NO. tspd20210317)
    • Principle Award Recipient: HuXiaoke
  • the National Natural Science Foundation of China (Award No. 92051119)
    • Principle Award Recipient: HuXiaoke
  • Special Foundation of Science and Technology Resources Survey (Award 2019FY100700)
    • Principle Award Recipient: HuXiaoke
© 2025 The Authors
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References

  1. Gauthier G, Gauthier M, Christen R. Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 1995; 45:755–761 [View Article] [PubMed]
     [Google Scholar]
  2. Colarusso A, Lauro C, Calvanese M, Parrilli E, Tutino ML. Active human full-length CDKL5 produced in the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125. Microb Cell Fact 2022; 21:211 [View Article] [PubMed]
     [Google Scholar]
  3. Cuny H, Offret C, Boukerb AM, Parizadeh L, Lesouhaitier O et al. Pseudoalteromonas ostreae sp. nov., a new bacterial species harboured by the flat oyster Ostrea edulis. Int J Syst Evol Microbiol 2021; 71: [View Article] [PubMed]
     [Google Scholar]
  4. Wen J, Liao L, Duan Z, Su S, Zhang J et al. Identification and regulatory roles of a new csr small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in temperature responses. Microbiol Spectr 2023; 11:e0409422 [View Article] [PubMed]
     [Google Scholar]
  5. Rahmani A, Parizadeh L, Baud M, Francois Y, Bazire A et al. Potential of marine strains of Pseudoalteromonas to improve resistance of Juvenile Sea Bass to pathogens and limit biofilm development. Probiotics & Antimicro Prot 2023 [View Article]
     [Google Scholar]
  6. Offret C, Rochard V, Laguerre H, Mounier J, Huchette S et al. Protective efficacy of a Pseudoalteromonas strain in European abalone, haliotis tuberculata, infected with Vibrio harveyi ORM4. Probiotics & Antimicro Prot 2019; 11:239–247 [View Article]
     [Google Scholar]
  7. Li H, Gao J, Ma S, Xiao R, Zhou X et al. Isolation and genome sequencing of a novel lytic Pseudoalteromonas phage SL20. Mar Genomics 2023; 71:101048 [View Article] [PubMed]
     [Google Scholar]
  8. Paulsen SS, Isbrandt T, Kirkegaard M, Buijs Y, Strube ML et al. Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov. Sci Rep 2020; 10:21630 [View Article] [PubMed]
     [Google Scholar]
  9. Hwang CY, Cho BC, Kang JK, Park J, Hardies SC. Genomic analysis of two cold-active Pseudoalteromonas phages isolated from the continental shelf in the Arctic ocean. Viruses 2023; 15:2061 [View Article] [PubMed]
     [Google Scholar]
  10. Sun X-H, Chen X-L, Wang X-F, Zhang X-R, Sun X-M et al. Cost-effective production of alginate oligosaccharides from Laminaria japonica roots by Pseudoalteromonas agarivorans A3. Microb Cell Fact 2023; 22:179 [View Article] [PubMed]
     [Google Scholar]
  11. Kim SJ, Youn UJ, Kang P, Kim TK, Kim I-C et al. A novel exopolysaccharide (p-CY01) from the Antarctic bacterium Pseudoalteromonas sp. strain CY01 cryopreserves human red blood cells. Biomater Sci 2023; 11:7146–7157 [View Article] [PubMed]
     [Google Scholar]
  12. Ju Y, Shan K, Liu W, Xi C, Zhang Y et al. Effect of different initial fermentation pH on exopolysaccharides produced by Pseudoalteromonas agarivorans Hao 2018 and identification of key genes involved in exopolysaccharide synthesis via transcriptome analysis. Mar Drugs 2022; 20:89 [View Article] [PubMed]
     [Google Scholar]
  13. Brass HUC, Klein AS, Nyholt S, Classen T, Pietruszka J. Condensing enzymes from Pseudoalteromonadaceae for prodiginine synthesis. Adv Synth Catal 2019; 361:2659–2667 [View Article]
     [Google Scholar]
  14. Wang J, Yin X, Xu M, Chen Y, Ji N et al. Isolation and characterization of a high-efficiency algicidal bacterium Pseudoalteromonas sp. LD-B6 against the harmful dinoflagellate Noctiluca scintillans. Front Microbiol 2022; 13:1091561 [View Article]
     [Google Scholar]
  15. Deng Y, Wang X, Xu H, Liu C, Li R et al. Optimization of κ-selenocarrageenase production by Pseudoalteromonas sp. Xi13 and its immobilization. Molecules 2022; 27:7716 [View Article] [PubMed]
     [Google Scholar]
  16. Eze OC, Berebon DP, Emencheta SC, Evurani SA, Okorie CN et al. Therapeutic potential of marine probiotics: a survey on the anticancer and antibacterial effects of Pseudoalteromonas spp. Pharmaceuticals 2023; 16:1091 [View Article] [PubMed]
     [Google Scholar]
  17. Rathbun MJ. Descriptions of New Species of Crabs of the Family Ocypodidae Washington: 1913 pp 615–620
     [Google Scholar]
  18. Gürtler V, Stanisich VA. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 1996; 142 (Pt 1):3–16 [View Article] [PubMed]
     [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  20. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24:1596–1599 [View Article] [PubMed]
     [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
     [Google Scholar]
  22. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20:265–272 [View Article] [PubMed]
     [Google Scholar]
  23. Li R, Li Y, Kristiansen K, Wang J. SOAP: short oligonucleotide alignment program. Bioinformatics 2008; 24:713–714 [View Article] [PubMed]
     [Google Scholar]
  24. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
     [Google Scholar]
  25. Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJM et al. ABySS: a parallel assembler for short read sequence data. Genome Res 2009; 19:1117–1123 [View Article] [PubMed]
     [Google Scholar]
  26. Yoon S-H, Ha S, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article] [PubMed]
     [Google Scholar]
  27. Auch AF, von Jan M, Klenk H-P, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article] [PubMed]
     [Google Scholar]
  28. Meier-Kolthoff JP, Klenk H-P, Göker M. Taxonomic use of DNA G+C content and DNA-DNA hybridization in the genomic age. Int J Syst Evol Microbiol 2014; 64:352–356 [View Article] [PubMed]
     [Google Scholar]
  29. Park S, Won SM, Kim H, Park DS. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microb 2024; 64:2969–2974 [View Article]
     [Google Scholar]
  30. Yin Q, Song Z-M, Liang J, Wang Y, Zheng X et al. Pseudooceanicola onchidii sp. nov., isolated from a marine invertebrate from the South China Sea. Int J Syst Evol Microbiol 2020; 70:1224–1230 [View Article] [PubMed]
     [Google Scholar]
  31. Hwang CY, Kim MH, Bae GD, Zhang GI, Kim YH et al. Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda. Int J Syst Evol Microbiol 2009; 59:1856–1861 [View Article] [PubMed]
     [Google Scholar]
  32. Luo P, Wang W, Wang Y, Hu C, He X. Pseudoalteromonas xishaensis sp. nov., isolated from Acanthaster planci in the Xisha islands. Antonie Van Leeuwenhoek 2013; 104:779–785 [View Article] [PubMed]
     [Google Scholar]
  33. Ying Y, Tian X-X, Wang J-J, Qu L-Y, Li J. Pseudoalteromonas fenneropenaei sp. nov., a marine bacterium isolated from sediment of a Fenneropenaeus chinensis pond. Int J Syst Evol Microbiol 2016; 66:2754–2759 [View Article]
     [Google Scholar]
  34. Park S, Lee SY, Kim W, Yoon JH. Pseudoalteromonas caenipelagi sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2020; 70:6301–6306 [View Article]
     [Google Scholar]
  35. Hwang CY, Lee I, Hwang YJ, Yoon SJ, Lee WS et al. Pseudoalteromonas neustonica sp. nov., isolated from the sea surface microlayer of the Ross sea (Antarctica), and emended description of the genus Pseudoalteromonas. Int J Syst Evol Microbiol 2016; 66:3377–3382 [View Article] [PubMed]
     [Google Scholar]
  36. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. In MIDI Technical Note 101, MIDI Inc, Newark, DE 1990
     [Google Scholar]
  37. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  38. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article] [PubMed]
     [Google Scholar]
  39. Ivanova EP, Sawabe T, Lysenko AM, Gorshkova NM, Svetashev VI et al. Pseudoalteromonas ruthenica sp. nov., isolated from marine invertebrates. Int J Syst Evol Microbiol 2002; 52:235–240 [View Article]
     [Google Scholar]
  40. Chun J, Rainey FA. Integrating genomics into the taxonomy and systematics of the bacteria and archaea. Int J Syst Evol Microbiol 2014; 64:316–324 [View Article] [PubMed]
     [Google Scholar]
  41. Navarro-Torre S, Carro L, Rodríguez-Llorente ID, Pajuelo E, Caviedes MÁ et al. Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation. Int J Syst Evol Microbiol 2020; 70:3287–3294 [View Article] [PubMed]
     [Google Scholar]
  42. Zhao C-H, Luo J-J, Gong T, Huang X-L, Ye D-Z et al. Pseudoalteromonas xiamenensis sp. nov., a marine bacterium isolated from coastal surface seawater. Int J Syst Evol Microbiol 2014; 64:444–448 [View Article] [PubMed]
     [Google Scholar]
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Pseudoalteromonas qingdaonensis sp. nov., isolated from the intestines of Ilyoplax deschampsi
Int J Syst Evol Microbiol 75, 006625 (2025); https://doi.org/10.1099/ijsem.0.006625
/content/journal/ijsem/10.1099/ijsem.0.006625
/content/journal/ijsem/10.1099/ijsem.0.006625
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