RESULTS:
1 - 5 of 5 for ""rotten wood""
Candida yunnanensis sp. nov. and Candida parablackwelliae sp. nov., two yeast species in the Candida albicans/Lodderomyces clade
During studies on the yeast communities associated with rotting wood in the Xishuangbanna Tropical Rainforest in PR China four novel yeast strains were found. Phylogenetic analysis based on the concatenated sequences of the D1/D2 domains of the large subunit rRNA gene and the ITS regions showed that these strains represented two novel species in the Candida albicans/Lodderomyces clade. The novel species represented by strains NYNU 17948 and NYNU 17981 formed a clade with Candida maltosa and Candida baotianmanensis with 1–1.8% sequence divergence in the D1/D2 domains and 8.9–10% sequence divergence in the ITS regions. The other novel species represented by NYNU 17105 and NYNU 17763 is most closely related to Candida blackwelliae with 0.7 % sequence divergence in the D1/D2 domains and 6.9 % sequence divergence in the ITS regions. The two novel species could be distinguished from their closest described species in terms of physiological traits. The two novel species are described as Candida yunnanensis sp. nov. (holotype NYNU 17948) and Candida parablackwelliae sp. nov. (holotype NYNU 17763).
Deakozyma yunnanensis sp. nov., a novel yeast species isolated from rotten wood
Three strains representing a novel yeast species were isolated from rotten wood samples collected in Xishuangbanna Tropical Rainforest in Yunnan Province PR China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions indicated that the novel species represented a member of the genus Deakozyma. The novel species was related most closely to the type strain of Deakozyma indianensis but they differed by 5.8 % sequence divergence (20 substitutions and 5 gaps) in the D1/D2 domain and by 12.2 % sequence divergence (27 substitutions and 15 gaps) in the ITS region. Interestingly the novel yeast species was able to ferment glucose and sucrose in Durham tubes a clear difference from its closest relative D. indianensis the type species of the genus Deakozyma. The species name of Deakozyma yunnanensis sp. nov. is proposed to accommodate these strains with NYNU 16742T (=CICC 33160T=CBS 14688T) designated the type strain. As the current description of the genus does not allow the inclusion of sugar fermenting species the emendation of the diagnosis of the genus Deakozyma Kurtzman and Robnett is proposed.
Vanrija jinghongensis sp. nov., an asexual basidiomycetous yeast from rotting wood
Three strains of a novel basidiomycetous yeast were isolated from the Xishuangbanna Tropical Rainforest Yunnan Province PR China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions indicated that the novel species represents a member of the genus Vanrija. It differed from the most closely related known species Vanrija albida CBS 2839T by 1.5 % sequence divergence (seven substitutions and two gaps out of 597 bp) in the D1/D2 domains and by 7.4 % sequence divergence (17 substitutions and 20 gaps over 495 bp) in the ITS regions respectively. The three strains of the novel species reproduced asexually and no mating could be found. In contrast to V. albida the novel yeast species was able to assimilate d-glucosamine inulin erythritol and galactitol and unable to assimilate raffinose. The name Vanrija jinghongensis sp. nov. is proposed to accommodate these strains with NYNU 17910T (=CICC 33269=CBS 15229) as the type strain.
Wickerhamomyces menglaensis f.a., sp. nov., a yeast species isolated from rotten wood
Five strains NUNU 16637 NYNU 16645 NYNU 1673 NYNU 1680 and NYNU 1689 of a novel ascomycetous yeast were isolated from the Xishuangbanna tropical rainforest Yunnan Province PR China. The five strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions. Sequence analysis showed that they represent undescribed yeast species belonging to the genus Wickerhamomyces. They differed from their closest known species Wickerhamomyces xylosivorus NBRC 111553T by 3.4 % sequence divergence (14 substitutions and six gaps out of 584 bp) in the D1/D2 domains and by 9.6 % sequence divergence (28 substitutions and 24 gaps over 543 bp) in the ITS regions respectively. The five strains of novel species reproduced asexually; no sexual reproduction could be found. In contrast to W. xylosivorus the novel yeast species were able to assimilate l-arabinose inulin soluble starch d-mannitol and citrate and unable to assimilate trehalose raffinose 5-keto-d-gluconate d-gluconate ethanol ethylamine and cadaverine. Growth was observed at 35 °C. The name Wickerhamomyces menglaensis f.a. sp. nov. is proposed to accommodate these strains with NYNU 1673 as the holotype.
Blastobotrys baotianmanensis sp. nov. and Blastobotrys xishuangbannaensis f.a., sp. nov., two novel yeast species associated with insects and rotting wood
Five yeast strains were isolated from the gut of the groundbeetle Pterostichus gebleri and rotting wood which were collected from two different localities in China. These strains were identified as representing two novel species of the genus Blastobotrys through comparison of sequences in the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. Blastobotrys baotianmanensis sp. nov. produces two to three spherical ascospores per ascus and is most closely related to the type strains of B. elegans B. capitulata B. arbuscula and an undescribed species represented by strain BG02-7-20-006A-3-1. Blastobotrys baotianmanensis sp. nov. differed from these strains by 3.6–8.4 % divergence (21–46 substitutions and 0–4 gaps) in the D1/D2 sequences. Blastobotrys xishuangbannaensis f.a. sp. nov. is closely related to B. nivea B. elegans and B. aristata but the formation of ascospores was not observed on various sporulation media and it differed from its relatives by 6.2–8.5 % divergence (34–43 substitutions and 2–6 gaps) in the D1/D2 sequences. The holotype of Blastobotrys baotianmanensis sp. nov. is NYNU 1581 and the holotype of Blastobotrys xishuangbannaensis f.a. sp. nov. is NYNU 181030.