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Pichia chibodasensis sp. nov., isolated in Indonesia
Three strains (14Y260T 14Y268 and 14Y276) of xylose-assimilating yeasts were isolated from decayed wood and soil collected in West Java in Indonesia. A phylogenetic analysis was performed based on the sequences of the D1/D2 domains of LSU SSU and EF-1α and the three strains were found to belong to the genus Pichia. The morphological biochemical physiological and chemotaxonomic characteristics indicated that these strains were distinct from other closely related species. Strains 14Y260T 14Y268 and 14Y276 belonged to the Pichia clade and represent a novel species named Pichia chibodasensis sp. nov. ; The type strain is 14Y260T (=NBRC 111569T=InaCC Y1042T).
Pichia kurtzmaniana f.a. sp. nov., with the transfer of eight Candida species to Pichia
Three yeast strains belonging to the ascomycetous yeast genus Pichia were isolated from two soil samples from Yunnan and Guizhou provinces and a marine water sample from Liaoning province PR China. Phylogenetic analyses based on the sequences of the D1/D2 domains of the large subunit(LSU) rRNA gene and the internal transcribed spacer (ITS) region indicate that these three strains together with 12 additional strains isolated from various substrates collected in different regions or countries of the world represent a novel species of the genus Pichia for which the name Pichia kurtzmaniana sp. nov. (holotype: strain CGMCC 2.7213) is proposed. The novel species differs from its close relatives Candida californica by eight (1.5 %) and 26 (11.1 %) mismatches in the D1/D2 domains and the ITS region respectively; and from Pichia chibodasensis by 11 (2.1 %) and 20 (8.7 %) mismatches in the D1/D2 domains and the ITS region respectively. In addition eight Candida species which belong to the Pichia clade are transferred to the genus Pichia resulting in the proposal of the following new combinations: Pichia cabralensis comb. nov. Pichia californica comb. nov. Pichia ethanolica comb. nov. Pichia inconspicua comb. nov. Pichia phayaonensis comb. nov. Pichia pseudolambica comb. nov. Pichia rugopelliculosa comb. nov. and Pichia thaimueangensis comb. nov.