RESULTS:
1 - 20 of 20 for ""Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand""
Pichia bovicola sp. nov., a yeast species associated with small-intestinal content of cattle
Three yeast strains DMKU-MP6-4T DMKU-MP2-6 and DMKU-MP5-1 were isolated from the small-intestinal content or Pia of cattle in Thailand during the investigation of yeast diversity in this habitat. According to the D1/D2 domain of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region sequence analysis these strains represent a novel yeast species in the genus Pichia. The species produced one to four ascospores per ascus with spherical to ellipsoidal shape and heterogenous in terms of size. These three strains were identical and differed from their closely related species Pichia exigua NRRL Y-10920T by 2% (six nucleotide substitutions and five gaps) in the D1/D2 domain of the large subunit rRNA gene while ITS region sequences differed by 3.1% (16 nucleotide substitutions and 27 gaps) 3.7% (19 nucleotide substitutions and 28 gaps) and 3.1% (16 nucleotide substitutions and 27 gaps) for DMKU-MP6-4T DMKU-MP2-6 and DMKU-MP5-1 respectively. The name Pichia bovicola is proposed to accommodate these species. The holotype is DMKU-MP6-4T (TBRC 15616T=PYCC 8905T).
Exophiala amphawaensis sp. nov., a novel black yeast isolated from the gut of amphipods in Thailand
The genus Exophiala is polymorphic able to transition between yeast hyphal and pseudohyphal forms. Species of the genus Exophiala are ubiquitous fungi that are distributed in various environments around the world. During a survey of fungal diversity in the gut of amphipods (Floresorchestia amphawaensis and undescribed Dogielinotid amphipods) from the Amphawa estuary Samut Songkhram province Thailand five black yeast strains (DMKU-MG01 DMKU-MG07 DMKU-MG08 DMKU-HG10 and DMKU-FG04) were identified as representing a novel taxon on the basis of a combination of morphological and molecular phylogenetic features. The five strains did not produce filamentous hyphae or pseudohyphae. Only budding yeast cells were observed. On the basis of the phenotypic characteristics and the results of molecular analyses of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region the five strains were identified as representing a novel species via applied nucleotide pairwise analysis. They differed from the most closely related species Exophiala alcalophiala by 3.54 % nucleotide substitutions (20 nucleotide substitutions in 572 bp) in the D1/D2 domains of the LSU rRNA gene. Moreover the sequences of the ITS region of the five strains differed from those of the most closely related species E. alcalophiala by 7.44–9.62 % nucleotide substitutions and Exophiala halophiala by 7.2–7.53 % nucleotide substitutions. The results of phylogenetic analyses based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene confirmed that the five black yeast strains represented a single novel species of the genus Exophiala. In this study Exophiala amphawaensis sp. nov. is proposed to accommodate these strains. The holotype is TBRC 15626T and the isotype is PYCC9020. The MycoBank accession number of the novel species is MB 851477.
Vishniacozyma siamensis sp. nov., a new anamorphic tremellomycetous yeast species isolated from a mangrove forest in Thailand
Six strains (DMKU-SG26 DMKU-SG42 DMKU-SYM22 DMKU-RG41 DMKU-RX317 and DMKU-RGM25) representing a novel basidiomycetous yeast species were isolated from leaf surfaces of mangrove plants collected in Thailand. Pairwise sequence analysis indicated that the six strains either had identical nucleotide substitution in the D1/D2 domains of the large subunit (LSU) rRNA gene sequences or differed by one to three nucleotide(s). They also had identical or differed by one to five nucleotide substitution(s) in the internal transcribed spacer (ITS) regions. blastn searches of the GenBank database revealed that the six strains were closely related to the holotype of type strains of Vishniacozyma peneaus V. terrae V. phoenicis V. taiwanica and V. europaea but with 6–15 (1.14–2.48%) and 16–26 (5.4–8.8%) nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and the ITS regions respectively. Phylogenetic analysis based on the concatenated sequences of the ITS regions and D1/D2 domains of the LSU rRNA gene showed that these strains are placed in the Vishniacozyma clade but were at a distinctly different position from the other recognized species of the genus. Based on the phylogenetic analysis and phenotypic characteristics these six strains are a novel species of the genus Vishniacozyma for which the name Vishniacozyma siamensis sp. nov. is proposed to accommodate them. The holotype is TBRC 18499T and the ex-type culture is PYCC 10042 (=DMKU-SG26). The MycoBank number of the novel species is MB 855838.
Savitreea pentosicarens gen. nov., sp. nov., a yeast species in the family Saccharomycetaceae isolated from a grease trap
Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene the D1/D2 domain of the LSU rRNA gene the ITS region translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence Savitreea pentosicarens gen. nov. sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).
Teunia siamensis f.a., sp. nov., a novel tremellaceous yeast species isolated from soil in a secondary peat swamp forest area
The strain DMKU-XD44 representing an anamorphic novel yeast species was isolated from soil collected in a peat swamp forest (PSF) area in Rayong Botanical Garden in eastern Thailand. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region Teunia cuniculi CBS 10309T was the most closely related species. The novel species differed from the T. cuniculi type by 2.5 % (14 nucleotide substitutions) in the D1/D2 region of the LSU rRNA gene and by 8.0 % (40 nucleotide substitutions) in the ITS region. The results of a phylogenetic analysis based on the combined sequences of the ITS region and the D1/D2 region indicated that DMKU-XD44 represents a member of the Teunia clade in the Cryptococcaceae (Tremellales Tremellomycetes Agaricomycotina and Basidiomycota) and is phylogenetically distinct from other species of the genus Teunia in the clade. Therefore DMKU-XD44 represents a novel species of the genus Teunia. The name Teunia siamensis f.a. sp. nov. is proposed. The holotype is DMKU-XD44 while the MycoBank number is MB 832816.
Magnusiomyces siamensis sp. nov., a yeast-like fungus isolated from food waste
Three yeast strains DMKU-GTSP8-6 DMKU-GTSP8-14T and DMKU-JED8-73 were isolated from food waste in Thailand. Based on the phenotypic characteristics and sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region it was revealed that the three strains clustered with the Magnusiomyces/Saprochaete clade. These strains were distinguished from the closely related species Saprochaete quercus CBS 750.85 Magnusiomyces ovetensis CBS 192.55T Magnusiomyces starmeri CBS 780.96T Saprochaete chiloensis CBS 8187T and Magnusiomyces ingens CBS 517.90T by 11.4 13.1 11.9 11.2 and 12.6 % sequence divergence in the D1/D2 domain and by 34.6 34.5 33.6 33.2 and 34.9 % sequence divergence in the ITS region respectively. The new species which does not produce ascospores is described as Magnusiomyces siamensis. The holotype of Magnusiomyces siamensis is TBRC 15056T and the isotypes are DMKU-GTSP8-14T and PYCC 9023T.
Torulaspora nypae sp. nov., a novel yeast species isolated from nipa (Nypa fruticans Wurmb.) inflorescence sap in southern Thailand
Two strains (YSP-384 and YSP-399) representing a novel Torulaspora species were isolated from two nipa inflorescence sap samples collected in Trang province in the southern part of Thailand. The two strains had identical sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions. The two strains were closest to Torulaspora maleeae CBS 10694T but with 1.1 % nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 5.2 % nucleotide substitutions in the ITS regions. Phylogenetic analysis based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene supported that the two strains represented a distinct species in the genus Torulaspora. Some phenotypic characteristics of the two strains differed from T. maleeae including the two strains have ability to assimilate d-xylose d-glucono-δ-lactone and melizitose and inability to ferment maltose and raffinose whereas T. maleeae has opposite results. Therefore the two strains are described as representing a novel species for which the name Torulaspora nypae sp. nov. was proposed.
Kodamaea samutsakhonensis f.a., sp. nov., a novel ascomycetous yeast species isolated from wild mushrooms in Thailand
Two strains of genus Kodamaea representing a novel anamorphic yeast species were isolated from two samples of Marasmiellus sp. collected in Thailand. Analysis of the sequences of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit (LSU) rRNA gene showed that the two strains differed by 27–42 nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 7–34 nucleotide substitutions in the ITS region of a group of related species Kodamaea smagusa CBS 11430T Kodamaea fungicola JCM 10142T Kodamaea plutei ATCC MYA-4329T Kodamaea lidongshanica SD5S01T and Kodamaea jinghongensis NYNU 167162T. Phylogenetic analysis based on the concatenated sequences of the ITS and the D1/D2 domains of the LSU rRNA gene showed that the two strains were placed in the Kodamaea clade and clearly separated from other recognized species of the genus. Therefore the two strains were assigned as a novel species of the genus Kodamaea for which we propose the name Kodamaea samutsakhonensis f.a. sp. nov. The holotype is TBRC 16043T (=DMKU-BP19T) and the isotype is PYCC 9354. The MycoBank number of the novel species is MB 846490.
Yamadazyma oleae f.a. sp. nov. and Yamadazyma molendinolei f.a. sp. nov., two novel ascomycetous yeast species isolated from olive oil mills in Italy, and reassignment of 11 Candida species to the genus Yamadazyma
Three yeast strains were isolated from an olive paste sample (CBS 18661) and two samples of olive oil (CBS 18662 and CBS 18660) were collected in two different mills in Verona province Italy. The sequence comparison of the D1/D2 domains of the LSU rRNA gene and the internal transcribed space (ITS) regions indicated that these strains belonged to the genus Yamadazyma. Phylogenetic analysis of concatenated sequences of the ITS regions and the D1/D2 domains indicated that they represented members of two distinct species. Strain CBS 18661 and CBS 18662 showed sequence divergence of 2.7–2.8% (13–15 nucleotide substitutions) in the D1/D2 domains from the holotype of Yamadazyma luoyangensis Yamadazyma ovata Yamadazyma siamensis and Candida kanchanaburiensis and 5.9–6.2% identities (36–38 nucleotide substitutions) in the ITS regions from Yamadazyma akitaenisis and Yamadazyma nakazawae. Strain CBS 18660 showed sequence divergence of 0.75–1.1% (4–6 nucleotide substitutions) from Y. ovata Candida trypodendri and Candida insectorum and 3.2–4.1% (18–24 nucleotide substitutions) in the ITS regions from Yamadazyma dushanensis Yamadazyma terventina Yamadazyma mexicana and C. trypodendri. The strains CBS 18661T (PP391581-PP375117) and CBS 18662 (PP391582-PP375118) are named Yamadazyma oleae f.a. sp. nov. whereas CBS 18660T (PP149061-PP130150) is assigned as Yamadazyma molendinolei f.a. sp. nov. The MycoBank numbers are MB 854683 and MB 854684 respectively. In addition 11 Candida species belonging to the Yamadazyma clade have been reassigned to the genus Yamadazyma with the proposal of the following new combinations: Yamadazyma aaseri comb. nov. Yamadazyma conglobata comb. nov. Yamadazyma dendronema comb. nov. Yamadazyma diddensiae comb. nov. Yamadazyma germanica comb. nov. Yamadazyma insectorum comb. nov. Yamadazyma kanchanaburiensis comb. nov. Yamadazyma naeodendra comb. nov. Yamadazyma pseudoaaseri comb. nov. Yamadazyma trypodendri comb. nov. and Yamadazyma vaughaniae comb. nov.
Metahyphopichia suwanaadthiae sp. nov., an anamorphic yeast species in the order Saccharomycetales and reassignment of Candida silvanorum to the genus Metahyphopichia
Seven yeast strains representing a single novel anamorphic species were isolated in Thailand. They consisted of five strains (DMKU-MRY16T DMKU-SK18 DMKU-SK25 DMKU-SK30 and DMKU-SK32) obtained from five different mushrooms and two strains (ST-224 and 11-14.2) derived from insect frass and soil respectively. The pairwise sequence analysis indicated that all seven strains had identical sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. Metahyphopichia silvanorum was the most closely related species but with 11.9–12.4% nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 13.1–13.3% nucleotide substitutions in the ITS region. The phylogenetic analyses based on the concatenated sequences of the ITS region and the D1/D2 domains of the LSU rRNA gene showed that the seven strains form a well-separated subclade in a clade containing M. silvanorum and Metahyphopichia laotica with high bootstrap support. A phylogenetic analysis of a multilocus dataset including the small subunit (SSU) rRNA gene the ITS region the D1/D2 domains of the LSU rRNA gene translation elongation factor 1-alpha gene actin gene and the RNA polymerase II subunit 2 gene confirmed the presence of the monophyletic clade that also includes M. silvanorum and M. laotica and strongly supported the phylogenetic isolation of the seven strains from its neighbouring species. Therefore the seven strains were assigned as a single novel species of the genus Metahyphopichia according to their phylogenetic relationships. The name Metahyphopichia suwanaadthiae sp. nov. is proposed to accommodate the seven strains. The holotype is DMKU-MRY16T (TBRC 11775T=NBRC 114386T=PYCC 8655T). The MycoBank number of the novel species is MB 841280. In addition Candida silvanorum is reassigned to the genus Metahyphopichia. The MycoBank number of M. silvanorum comb. nov. is MB 841279.
Kazachstania surinensis f.a., sp. nov., a novel yeast species isolated from Thai traditional fermented food
Seven yeast strains (UBIF12-1 UBFB13-1 SRFS56-3 SRFS57-2 SKFS62-1 SKFS66-1 and SKFS67-1) representing a single anamorphic novel yeast species were isolated from traditional Thai fermented foods in Ubon Ratchathani Surin and Sisaket in the northeast part of Thailand. The results of analysis of the sequences of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the seven strains showed zero to one nucleotide substitutions in the sequences of the D1/D2 region of the LSU rRNA gene and zero to four nucleotide substitutions in the ITS region. Kazachstania humilis CBS 5658T was the most closely-related species but with 0.7–0.9% nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 2.0–2.2% nucleotide substitution in the ITS region. The results of a phylogenetic analysis based on the concatenated ITS and D1/D2 regions confirmed that the seven strains represented a single species of the genus Kazachstania distinct from the other recognized species of the genus. Furthermore the morphological biochemical and physiological properties of the seven strains not only indicated that they represented members of the genus Kazachstania but that they were separated from K. humilis and K. pseudohumilis the two most closely related species in the phylogenetic tree. Therefore the seven strains were identified as representing a novel species for which we propose the name Kazachstania surinensis f.a. sp. nov. The holotype is TBRC 15053T (isotype: SRFS57-2 and PYCC 9021). The MycoBank number of the novel species is 841892.
Spencerozyma pingqiaoensis sp. nov., a yeast species isolated from the external surface of rice leaves in China
Four strains (NYNU 178247 NYNU 178251 DMKU-PAL160 and DMKU-PAL137) representing a novel yeast species were isolated from the external surfaces of rice and pineapple leaves collected in China and Thailand. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit rRNA gene revealed that the novel species belonged to the genus Spencerozyma. The D1/D2 sequence of the novel species differed from its closest relative Spencerozyma acididurans SYSU-17T by 3.2 % sequence divergence. The species also differed from Spencerozyma crocea CBS 2029T and Spencerozyma siamensis DMKU13-2T by 3.0–6.9 % sequence divergence in the D1/D2 sequences out of 592 bp. In the ITS regions the novel species displayed 19.8–29.2% sequence divergence from S. acididurans SYSU-17T S. crocea CBS 2029T and S. siamensis DMKU13-2T out of 655 bp. Furthermore the novel species could also be differentiated from the closely related species by some physiological characteristics. The species name of Spencerozyma pingqiaoensis sp. nov. (Holotype CBS 15238 Mycobank MB 844734) is proposed to accommodate these four strains.
Komagataeibacter diospyri sp. nov., a novel species of thermotolerant bacterial nanocellulose-producing bacterium
Thermotolerant bacterial nanocellulose-producing strains designated MSKU 9T and MSKU 15 were isolated from persimmon and sapodilla fruits respectively. These strains were aerobic Gram-stain-negative had rod-shaped cells were non-motile and formed white–cream colonies. Phylogeny based on the 16S rRNA gene sequences revealed that MSKU 9T and MSKU 15 represented members of the genus Komagataeibacter and formed a monophyletic branch with K. swingsii JCM 17123T and K. europaeus DSM 6160T. The genomic analysis revealed that overall genomic relatedness index values of MSKU 9T with K. swingsii JCM 17123T and K. europaeus DSM 6160T were ~90 % average nucleotide identity (ANI) and ≤58.2 % digital DNA–DNA hybridization (dDDH) respectively. MSKU 9T and MSKU 15 can be differentiated from the closely related K. swingsii JCM 17123T by their growth on 30 % d-glucose and ability to utilize and to form acid from raffinose and sucrose as carbon sources and from K. europaeus DSM 6160T by their ability to grow without acetic acid. The genomic DNA G+C contents of MSKU 9T and MSKU 15 were 60.4 and 60.2 mol% respectively. The major fatty acids of MSKU 9T and MSKU 15 were summed feature 8 (C18 : 1 ω7c and/or C18 : 1ω6c). The respiratory quinone was determined to be Q10. On the basis of the results of the polyphasic taxonomic analysis MSKU 9T (=TBRC 9844T=NBRC 113802T) represents a novel species of the genus Komagataeibacter for which the name Komagataeibacter diospyri sp. nov. is proposed.
Donghicola mangrovi sp. nov., a member of the family Rhodobacteraceae isolated from mangrove forest in Thailand
Two novel Gram-stain-negative rod-shaped and non-motile bacterial strains designated B5-SW-15T and C2-DW-16 were isolated from water collected in mangrove forests in Ranong Province Thailand. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains B5-SW-15T and C2-DW-16 belonged to the genus Donghicola and were most closely related to Donghicola tyrosinivorans DSM 100212T (98.2 and 98.1 % respectively) and Donghicola eburneus DSM 29127T (97.7 and 97.6 % respectively). The average nucleotide identity and digital DNA–DNA hybridization values between strain B5-SW-15T strain C2-DW-16 and related species were 95.8 and 71.6 % (to strain C2-DW-16) 76.8 and 21.3 % (to D. tyrosinivorans DSM 100212T) and 80.3 and 24.2 % (to D. eburneus DSM 29127T) respectively. The predominant cellular fatty acids (>5 %) were summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) C16 : 0 and C12 : 1 3-OH. Ubiquinone Q-10 was the sole respiratory quinone. DNA G+C contents of the isolates were 61.0 and 61.2 mol% based on whole genome sequences. Strains B5-SW-15T and C2-DW-16 contained aminolipid phosphatidylcholine phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. On the basis of the results from phenotypic chemotaxonomic and phylogenetic analyses strains B5-SW-15T and C2-DW-16 constitute a novel species of the genus Donghicola in the family Rhodobacteraceae for which the name Donghicola mangrovi sp. nov. is proposed. The type strain is B5-SW-15T (=BCC 56522T=TBRC 9562T=KCTC 72743T).
Yamadazyma sisaketensis f.a., sp. nov. and Yamadazyma koratensis f.a., sp. nov., two novel ascomycetous yeast species from mushrooms and cocoa leaves in Thailand, and reassignment of Candida andamanensis, Candida jaroonii and Candida songkhlaensis to the genus Yamadazyma
Six strains representing two novel ascomycetous yeast species were isolated from mushroom fruiting bodies and cocoa leaves collected in Thailand. Analysis of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit rRNA gene sequences showed that the six strains were divided into two groups. The first group consisted of four strains (DMKU-SSK46 DMKU-SK1 SCCL3-5 and SCCL19-3) that were closely related to the type strains of Candida conglobata Candida insectorum Yamadazyma dushanensis Yamadazyma mexicana and Yamadazyma riverae but with 12–14 (2.5–2.9 %) and 28–50 (5.4–8.8 %) nucleotide substitutions in the D1/D2 domains and the ITS regions respectively. However two strains (DMKU-KMY40 and DMKU-KO18) of the second group differed from a group of described species Candida diddensiae Candida dendronema Candida germanica Candida kanchanaburiensis Candida naeodendra Candida vaughaniae and Yamadazyma siamensis by 8–15 (1.5–2.8 %) and 45–53 (8.2–9.6 %) nucleotide substitutions in the D1/D2 domains and the ITS regions respectively. Phylogenetic analysis based on the concatenated sequences of the ITS regions and D1/D2 domains showed that these strains represented two species of the Yamadazyma clade that were distinct from the other related species. Based on the phylogenetic analysis and phenotypic characteristics these six strains were assigned to two novel species of the genus Yamadazyma although formation of ascospores was not observed. Yamadazyma sisaketensis f.a. sp. nov. is proposed for the first group (four strains). The holotype is TBRC 17139T (ex-type culture: PYCC 9797). The MycoBank number is MB 849637. Yamadazyma koratensis f.a. sp. nov. is proposed for the second group (two strains). The holotype is TBRC 14868T (ex-type culture: PYCC 8907). The MycoBank number is MB 849638. In addition it is proposed that Candida andamanensis Candida jaroonii and Candida songkhlaensis are reassigned to the genus Yamadazyma as Yamadazyma andamanensis comb. nov. Y. jaroonii comb. nov. and Y. songkhlaensis comb. nov. respectively.
Starmerella fangiana f.a. sp. nov., a new ascomycetous yeast species from Daqu-making environment and other sources
In the survey of yeast diversity in high-temperature Daqu which is a fermentation starter for Chinese sauce-flavoured Baijiu six yeast strains representing one novel species of the genus Starmerella were isolated from samples of Daqu and surrounding environments collected in Zunyi city Guizhou Province China. Phylogenetic analyses of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene indicate that these six strains are conspecific with three other strains isolated from flowers and duckweed collected in Samoa India and Thailand. The representative strain QFC-8 of the new species differs from the closet species Starmerella caucasica resolved by the D1/D2 sequence analysis by 13 (3.1 % 12 substitutions and 1 gap) and 40 (10.3 % 9 substitutions and 31 gaps) mismatches in the D1/D2 domain and ITS region respectively. The results suggest that the novel group represents an undescribed species in the genus Starmerella for which the name Starmerella fangiana sp. nov. is proposed. The holotype strain is CGMCC 2.7773.
Phylogenomic delineation of two new species of ascomycetous yeasts, Wickerhamiella koratensis sp. nov. and Wickerhamiella limtongiae sp. nov., and proposal of two synonyms, Wickerhamiella infanticola and Wickerhamiella tropicalis
Two novel ascomycetous yeast species of the genus Wickerhamiella are proposed based on isolates obtained in Thailand from food waste and the fruiting body of a polypore fungus and on a combination of conventional DNA-barcode sequence analyses and whole-genome phylogenies. We focus on a particular subclade of the genus Wickerhamiella that contains species found in anthropic environments and describe Wickerhamiella limtongiae sp. nov. (DMKU-FW31-5T=PYCC 9022T=TBRC 15055T) found on food waste samples. In an adjacent clade we describe Wickerhamiella koratensis sp. nov. (DMKU-KO16T=PYCC 8908T=TBRC 14869T) which represents the closest relative of Wickerhamiella slavikovae and was isolated from the fruiting body of Bjerkandera sp. In the subclade of W. limtongiae sp. nov. we propose that Wickerhamiella infanticola should be regarded as a synonym of Wickerhamiella sorbophila and that Wickerhamiella tropicalis should be regarded as a synonym of Wickerhamiella verensis.
Wickerhamiella nakhonpathomensis f.a. sp. nov., a novel ascomycetous yeast species isolated from a mushroom and a flower in Thailand
Strains SU22T (TBRC 14875T) and FLA11.5 representing a novel anamorphic yeast species were respectively isolated from a fruiting body of a Coprinus species and an inflorescence of a Coffea species collected in Thailand. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions showed that the two strains differed by two nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and were identical in the ITS regions. Wickerhamiella drosophilae CBS 8459T was the most closely related species but with 24–26 nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 24 nucleotide substitutions in the ITS regions. A phylogenetic analysis based on the sequences of the D1/D2 domains indicated that the two strains represented a species in the genus Wickerhamiella which was distinct from other recognized species of the genus. Therefore the two strains were assigned as a novel species for which we propose the name Wickerhamiella nakhonpathomensis f.a. sp. nov. The holotype is TBRC 14875T (isotype PYCC 8914T). The MycoBank number of the novel species is MB 840833.
Savitreea siamensis sp. nov., an ascomycetous yeast species in the family Saccharomycetaceae discovered in Thailand
Four yeast strains representing a novel anamorphic species were isolated in Thailand. The two strains (ST-3660T and ST-3647) were obtained from two different estuarine water samples in a mangrove forest. Strain DMKU-FW1-37 was derived from a grease sample and another strain (TSU57) was isolated from a fruiting body of Phallus sp. Pairwise sequence analysis showed that the four strains had identical or differed by only one nucleotide substitution in the D1/D2 domains of the large subunit (LSU) rRNA gene and differed by one to three nucleotide substitutions in the internal transcribed spacer (ITS) regions. Savitreea pentosicarens is the most closely related species to the four strains but with 9–10 (1.57–1.72 %) nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 29–31 (4.22–4.45 %) nucleotide substitutions in the ITS regions. Phylogenetic analyses based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene showed that the four strains form a well-separated lineage from S. pentosicarens with high bootstrap support confirming that they represent a distinct species. Therefore the four strains are assigned as representives of a novel species of the genus Savitreea for which the name Savitreea siamensis sp. nov. is proposed. The holotype is TBRC 4481T and the ex-type is PYCC 9794T (=ST-3660T). The MycoBank number of the novel species is MB 851951.
Wickerhamiella martinezcruziae f. a., sp. nov., a yeast species isolated from tropical habitats
Four yeast isolates with an affinity to the genus Wickerhamiella were obtained from beach sand a marine zoanthid and a tree exudate at different localities in Brazil. Two other isolates with almost identical ITS and D1/D2 sequences of the large subunit rRNA gene were isolated from the small intestine of cattle and a grease trap in Thailand. These isolates represent a novel species phylogenetically related to Wickerhamiella verensis Wickerhamiella osmotolerans Wickerhamiella tropicalis Wickerhamiella sorbophila and Wickerhamiella infanticola. The novel species differs by 15–30 nucleotide differences from these species in the D1/D2 sequences. The name Wickerhamiella martinezcruziae f.a. sp. nov. is proposed. The holotype of Wickerhamiella martinezcruziae sp. nov. is CBS 16104T. The MycoBank number is MB 839328.