RESULTS:
1 - 4 of 4 for ""Rhodoferax""
Rhodoferax lacus sp. nov., isolated from a large freshwater lake
A Gram-staining-negative aerobic motile with a single polar flagellum and rod-shaped bacterium as a bacterial host of podovirus P26218 designated IMCC26218T was isolated from Lake Soyang South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC26218T belonged to the genus Rhodoferax of the family Comamonadaceae and shared 97.7–99.0 % sequence similarities with Rhodoferax species. The draft whole genome sequence of strain IMCC26218T was ca. 4.9 Mbp in size with the DNA G+C content of 62.3 mol%. Average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH) values between strain IMCC26218T and other Rhodoferax were 74.0–77.3 % and 19.5–21.0 % respectively showing that the strain represents a new Rhodoferax species. The strain contained summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and C16 : 0 as the major fatty acids and phosphatidylethanolamine three unidentified phospholipids two unidentified aminolipids and two unidentified lipids as major polar lipids. The predominant isoprenoid quinone of the strain was ubiquinone-8 (Q-8). On the basis of the phylogenetic and phenotypic characteristics strain IMCC26218T is considered to represent a novel species of the genus Rhodoferax for which the name Rhodoferax lacus sp. nov. is proposed. The type strain is IMCC26218T (=KACC 18983T=NBRC 112709T).
Rhodoferax bucti sp. nov., isolated from fresh water
A Gram-reaction-negative peach-brown-pigmented slightly curved-rod-shaped aerobic non-motile bacterium designated GSA243-2T was isolated from fresh water samples collected from the Chishui River flowing through Maotai Guizhou south-west PR China. Phenotypic chemotaxonomic and genomic traits were investigated. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Rhodoferax . The closest phylogenetic relative was Rhodoferax saidenbachensis ATCC BAA-1852T (98.35 %). The major fatty acids were C16: 0 and C16 : 1ω6c and/or C16 : 1ω7c. The major respiratory quinone was ubiquinone Q-8 and the major polar lipid was phosphatidylethanolamine. Genome sequencing revealed a genome size of 3.67 Mbp and a G+C content of 61.17 mol%. Pairwise-determined whole genome average nucleotide identity values and digital DNA–DNA hybridization values suggested that strain GSA243-2T represents a new species for which we propose the name Rhodoferax bucti sp. nov. with the type strain GSA243-2T (=CGMCC 1.16288T=KCTC 62564T).
Genomic insights into a novel species Rhodoferax aquaticus sp. nov., isolated from freshwater
A novel non-phototrophic member of the genus Rhodoferax was obtained from freshwater. The purpose of this study was to analyse the genome of a nonphototrophic strain and propose a new species based on its phylogenetic genomic physiological and chemotaxonomic characteristics. The results of phylogenetic analysis based on 16S rRNA gene sequences supports that the strain designated Gr-4T has a close relationship to the genus Rhodoferax . The observed average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain Gr-4T and its closest related strains were 72.3–74.6 % and 21.9–22.8 % respectively. These values were much lower than the species separation thresholds for ANI or dDDH of 95–96 and 70 % respectively and in fact fall in the intergeneric range. Strain Gr-4T does not contain RuBisCO-related genes but does contain GS/GOGAT pathway-related genes enabling nitrate ammonification. A polyphasic study and a genomic-level investigation were done to establish the taxonomic status of strain Gr-4T. Based on the phylogenetic genomic and physiological differences it is proposed that the isolate be classified to the genus Rhodoferax as Rhodoferax aquaticus sp. nov. with isolate Gr-4T (=KCTC 32394T=JCM 19166T) as the type strain.
Rhodoferax lithotrophicus sp. nov., a neutrophilic iron-oxidizing and -reducing bacterium isolated from iron-rich freshwater sediments
A neutrophilic iron-oxidizing and -reducing bacterium strain MIZ03T was previously isolated from a wetland in Ibaraki Japan. Here we report the detailed characteristics of this strain. It was motile with a single polar flagellum and Gram-stain-negative. It could grow not only chemolithoautotrophically but also chemoorganotrophically by aerobic respiration and fermentation. Major cellular fatty acids were C16 : 1 ω7c/C16 : 1 ω6c and C16 : 0. Phylogenetic analyses indicated that strain MIZ03T belonged to the genus Rhodoferax. This strain was closely related to Rhodoferax ferrireducens with 98.5 % of 16S rRNA gene sequence similarity. Based on its phenotypic and genomic based characteristics we conclude that strain MIZ03T represents a new species in the genus Rhodoferax. We propose the name Rhodoferax lithotrophicus sp. nov. to accommodate this strain. The type strain is MIZ03T (=JCM 34246T=DSM 113266T). We also propose the name Rhodoferax koreensis sp. nov. of which the type strain is DCY110T (=KCTC 52288T=JCM 31441T) for the effectively but not yet validly published name ‘Rhodoferax koreense’.