RESULTS:
1 - 5 of 5 for ""School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China""
Candida funiuensi sp. nov., a cellobiose-fermenting yeast species isolated from rotten wood
Two strains of an asexual cellobiose-fermenting yeast species were isolated from rotten wood samples collected in Funiu Mountain Nature Reserve in Henan Province central China. Molecular phylogenetic analysis that included the nearly complete small subunit (SSU) the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rDNA showed that these strains belonged to the Candida kruisii clade with Candida kruisii and Candida cretensis as their closest phylogenetic neighbours. The nucleotide differences between the novel strains and the type strains of C. kruisii and C. cretensis were 30 and 36 substitutions respectively in the D1/D2 LSU rDNA 40 and 44 substitutions respectively in the ITS region and 19 and 23 substitutions respectively in the SSU rDNA. The novel strains can also be distinguished from their closest described species C. kruisii and C. cretensis by a number of physiological characteristics and represent a novel species of the genus Candida for which the name Candida funiuensis sp. nov. is proposed. The type strain is NYNU 14625T ( = CICC 33050T = CBS 13911T). The Mycobank number is MB 811503.
Candida baotianmanensis sp. nov. and Candida pseudoviswanathii sp. nov., two ascosporic yeast species isolated from the gut of beetles
Four yeast strains were isolated from the gut of beetles collected on Baotianman Mountain and People's Park of Nanyang in Henan Province China. These strains produced unconjugated asci with one or two ellipsoidal to elongate ascospores in a persistent ascus. Phylogenetic analysis of the D1/D2 domains of the LSU rRNA gene sequences indicated that the isolates represent two novel sexual species in the Candida/Lodderomyces clade. Candida baotianmanensis sp. nov. was located in a statistically well-supported branch together with Candida maltosa. Candida pseudoviswanathii sp. nov. formed a subclade with its closest relative Candida viswanathii supported by a strong bootstrap value. The two novel species were distinguished from their most closely related described species Candida maltosa and Candida viswanathii in the D1/D2 LSU rRNA gene and internal transcribed spacer (ITS) sequences and in phenotypic traits. The type strain of Candida baotianmanensis sp. nov. is NYNU 14719T ( = CBS 13915T = CICC 33052T) and the type strain of Candida pseudoviswanathii sp. nov. is NYNU 14772T ( = CBS 13916T = CICC 33053T). The MycoBank numbers for Candida baotianmanensis sp. nov. and Candida pseudoviswanathii sp. nov. are MB 812621 and MB 812622.
Pichia dushanensis sp. nov. and Hyphopichia paragotoi sp. nov., two sexual yeast species associated with insects and rotten wood
Seven yeast strains were isolated from the gut of insect larvae and decayed wood which were collected from three localities near Nanyang Henan Province China. These strains were identified as two novel species through comparison of sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and other taxonomic characteristics. Pichia dushanensis sp. nov. was closely related to species in the Pichia clade and produced one to four spheroid ascospores in a deliquescent ascus. The D1/D2 sequence of P. dushanensis sp. nov. differed from its closest relative Issatchenkia (Pichia) sp. NRRL Y-12824 by 3.6 % sequence divergence (16 substitutions and 4 gaps). The species also differed from its four closest known species Candida rugopelliculosa Pichia occidentalis Pichia exigua and Candida phayaonensis by 4.1–4.4 % sequence divergence (22–24 substitutions and 0–2 gaps) in the D1/D2 sequences. Hyphopichia paragotoi sp. nov. belonged to the Hyphopichia clade and its nearest phylogenetic neighbours were Candida gotoi Candida pseudorhagii Candida rhagii and Hyphopichia heimii with 3.2–4.2 % sequence divergence (16–21substitutions and 1 gap) in the D1/D2 sequences. In comparison with previously established species H. paragotoi sp. nov. formed one hat-shaped ascospore in a persistent ascus. The type strain of P. dushanensis sp. nov. is NYNU 14658T ( = CICC 33049T = CBS 13912T) and the type strain of H. paragotoi sp. nov. is NYNU 14666T ( = CICC 33048T = CBS 13913T).
Wickerhamiella allomyrinae f.a., sp. nov., a yeast species isolated from the gut of the rhinoceros beetle Allomyrina dichotoma
Two strains representing Wickerhamiella allomyrinae f.a. sp. nov. were isolated from the gut of Allomyrina dichotoma (Coleoptera: Scarabeidae) collected from the Baotianman National Nature Reserve Nanyan Henan Province China. Sequence analyses of the D1/D2 domains of the LSU rRNA gene revealed that this novel species was located in the Wickerhamiella clade (Saccharomycetes Saccharomycetales) with three described species of the genus Candida namely Candida musiphila Candida spandovensis and Candida sergipensis as the most closely related species. The novel species differed from these three species by 9.3–9.8 % sequence divergence (35–45 nt substitutions) in the D1/D2 sequences. The species could also be distinguished from the closely related species C. musiphila C. spandovensis and C. sergipensis by growth on vitamin-free medium and at 37 °C. The type strain is Wickerhamiella allomyrinae sp. nov. NYNU 13920T ( = CICC 33031T = CBS 13167T).
Blastobotrys baotianmanensis sp. nov. and Blastobotrys xishuangbannaensis f.a., sp. nov., two novel yeast species associated with insects and rotting wood
Five yeast strains were isolated from the gut of the groundbeetle Pterostichus gebleri and rotting wood which were collected from two different localities in China. These strains were identified as representing two novel species of the genus Blastobotrys through comparison of sequences in the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. Blastobotrys baotianmanensis sp. nov. produces two to three spherical ascospores per ascus and is most closely related to the type strains of B. elegans B. capitulata B. arbuscula and an undescribed species represented by strain BG02-7-20-006A-3-1. Blastobotrys baotianmanensis sp. nov. differed from these strains by 3.6–8.4 % divergence (21–46 substitutions and 0–4 gaps) in the D1/D2 sequences. Blastobotrys xishuangbannaensis f.a. sp. nov. is closely related to B. nivea B. elegans and B. aristata but the formation of ascospores was not observed on various sporulation media and it differed from its relatives by 6.2–8.5 % divergence (34–43 substitutions and 2–6 gaps) in the D1/D2 sequences. The holotype of Blastobotrys baotianmanensis sp. nov. is NYNU 1581 and the holotype of Blastobotrys xishuangbannaensis f.a. sp. nov. is NYNU 181030.