RESULTS:
1 - 12 of 12 for ""novel yeast species""
Starmerella anomalae f.a., sp. nov., Starmerella asiatica f.a., sp. nov., Starmerella henanensis f.a., sp. nov. and Starmerella scarabaei f.a., sp. nov., four yeast species isolated from scarab beetles
Eleven yeast strains representing four novel species of the genus Starmerella were isolated from scarabs beetles collected in Nanyang Henan Province Central China. Phylogenetic analysis using combined sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions placed all the novel species in a clade distinct from the other known species in the clade representing the members of the genus Starmerella. These novel species differed from each other or their closest known species by more than 4.9 % nucleotide substitutions in the D1/D2 domains and by more than 9.1 % nucleotide substitutions in the ITS regions. The four novel species can also be separated from their closest relatives in terms of physiological characteristics. No asci or signs of conjugation were found in the novel species on the most common sporulation media. The novel species are designated as Starmerella anomalae f.a. sp. nov. (type strain NYNU 157145T=CICC 33094T=CBS 14178T) Starmerella asiatica f.a. sp. nov. (type strain NYNU 15782T=CICC 33089T=CBS 14173T) Starmerella henanensis f.a. sp. nov. (type strain NYNU 15766T=CICC 33088T=CBS 14172T) and Starmerella scarabaei f.a. sp. nov. (type strain NYNU 15821T=CICC 33090T=CBS 14174T).
Cyberlindnera tropicalis f.a., sp. nov., a novel yeast isolated from tropical regions
Two yeast strains DMKU-WBBC14 and UFMG-CM-Y3283 were isolated from soil in Samutprakarn province in the central part of Thailand and from rotting wood in an Amazonian forest site in the Roraima State in Brazil respectively. The two strains showed identical sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene and different sequences by three nucleotide substitutions of the internal transcribed spacer (ITS) region. Therefore these two strains represented a single species which was most closely related to Cyberlindnera mengyuniae CBS 10845T. The nucleotide sequence differences between the two strains of the novel species and the type strain Cyberlindnera mengyuniae CBS 10845T were 10 substitutions in the D1/D2 region of the LSU rRNA gene and 46 substitutions in the ITS region. DMKU-WBBC14 and UFMG-CM-Y3283 differed in growth temperature profiles. Moreover they also exhibited different carbon assimilation profiles and growth temperature profiles from the type strain of Cyberlindnera mengyuniae CBS 10845T. The name Cyberlindnera tropicalis f.a. sp. nov. is proposed. The type strain is DMKU-WBBC14T (=CBS 14558T=TBRC 6562T). The Mycobank number is MB 819722.
Sugiyamaella mastotermitis sp. nov. and Papiliotrema odontotermitis f.a., sp. nov. from the gut of the termites Mastotermes darwiniensis and Odontotermes obesus
Two novel yeast species were isolated from the guts of two different termite species. A new member of the genus Sugiyamaella was isolated from the hindgut and nest material of the lower Australian termite Mastotermes darwiniensis. The second novel yeast species isolated from the higher termite Odontotermes obesus was identified as a member of the genus Papiliotrema. Both yeast species were able to hydrolyse xylan methylumbelliferyl β-xylobiose and methylumbelliferyl β-xylotriose. The ability to debranch different hemicellulose side chains and growth without the addition of external vitamins was observed. A symbiotic role of the novel yeast species is indicated especially in respect to xylan degradation and the production of vitamins. Here we describe these species as Sugiyamaella mastotermitis sp. nov. MycoBank 816574 (type strain MD39VT=DSM 100793T=CBS 14182T) and Papiliotrema odontotermitis f.a. sp. nov. MycoBank 816575 (type strain OO5T=DSM 100791T=CBS 14181T). Additionally we transfer Candida qingdaonensis to the genus Sugiyamaella and propose the following combination: Sugiyamaella qingdaonensis f.a. comb. nov. MycoBank 816576.
Kurtzmaniella hittingeri f.a., sp. nov., isolated from rotting wood and fruits, and transfer of three Candida species to the genus Kurtzmaniella as new combinations
Twelve strains of a novel yeast species were isolated from rotting wood mushrooms and fruit samples in Brazil and French Guiana. Analysis of the sequences of the internal transcribed spacer region and the D1/D2 domains of the large subunit rRNA gene showed that the novel species belongs to the Kurtzmaniella clade. The novel species differed from its closest relative Candida natalensis by 12 substitutions in the D1/D2 sequences. The novel species could be distinguished from C. natalensis by its inability to assimilate cellobiose and salicin and growth at 50 % (w/w) glucose. The name Kurtzmaniella hittingeri f.a. sp. nov. is proposed for the novel species. The type strain of K. hittingeri sp. nov. is CBS 13469T (=UFMG CM-Y272T). The MycoBank number is 827183. We also propose the transfer of Candida fragi Candida quercitrusa and Candida natalensis to the genus Kurtzmaniella as new combinations.
Large biodiversity of yeasts in French Guiana and the description of Suhomyces coccinellae f.a. sp. nov. and Suhomyces faveliae f.a. sp. nov.
The extent of the diversity of yeasts in tropical rain forest and different environments from French Guiana was investigated. A total of 365 samples were collected from various substrates such as plants fruits and insects at 13 locations yielding 276 pure yeast isolates. Sequence analysis of the D1/D2 domains of the large subunit rRNA gene indicated that 210 isolates out of 276 belonged to 82 described species (67 Saccharomycotina 14 Basidiomycota and 1 Pezizomycotina). In addition to these a total of 54 Saccharomycotina isolates could not be assigned to a known species. These belonged to 14 genera and should be studied further from a taxonomic point of view. In addition among the 43 Basidiomycotina isolates found 12 could not be assigned to a known species. This report shows an unexpected biodiversity and indicates that oversea territories such as French Guiana constitute a largely unexplored reservoir for yeast diversity. Two Saccharomycotina strains CLIB 1706 and CLIB 1725 isolated from an insect and from a fern respectively were characterized further and were shown to belong to the Suhomyces clade on the basis of the rDNA sequence comparison. CLIB 1706TrDNA sequences showed nine substitutions and three indels out of 556 bp (D1/D2 domains) and 32 substitutions and 12 indels out of 380 bp [internal transcribed spacer (ITS)] with that of the most closely related species Suhomyces guaymorum CBS 9823T. CLIB 1725T rDNA sequences presented 18 substitutions and one indel out of 549 bp (D1/D2 domains) and 48 substitutions and 11 indels out of 398 bp (ITS) with that of its closest relative Suhomyces vadensis CBS 9454T. Two novel species of the genus Suhomyces were described to accommodate these two strains: Suhomyces coccinellae f.a. sp. nov. (CLIB 1706T=CBS 14298T) and Suhomyces faveliae f.a. sp. nov. (CLIB 1725T=CBS 14299T).
Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae
Seven yeast strains DMKU VGT1-14T DMKU VGT1-19T DMKU-JMGT1-28 DMKU-JMGT1-32 DMKU VGT2-06 DMKU VGT2-19 and DMKU VGT6-14 were isolated from a grease trap in Thailand and two strains SJ-1 and SN-102 were isolated from the sea surface microlayer in Taiwan. On the basis of phenotypic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region these strains represented two novel yeast species of the genus Wickerhamiella. In terms of pairwise sequence similarity four strains DMKU VGT1-14 DMKU-JMGT1-32 DMKU VGT6-14 and SN-102 were closely related to Wickerhamiella infanticola NRRL Y-17858T but differed by 13 nucleotide substitutions with one gap (2.46 %) in the D1/D2 domain of the LSU rRNA gene and 15 nucleotide substitutions with 23 gaps (4.2 %) in the ITS region. The strains DMKU VGT1-19T DMKU-JMGT1-28 DMKU VGT2-06 DMKU VGT2-19 and SJ-1 differed from the type strain of the most closely related species Wickerhamiella sorbophila NRRL Y-7921T by nine nucleotide substitutions with one gap (1.66 %) in the D1/D2 domain of the LSU rRNA gene and nine nucleotide substitutions with 17 gaps (2.52%) in the ITS region. Hence the names Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov. are proposed to accommodate these species in the genus Wickerhamiella. The holotypes are W. osmotolerans DMKU VGT1-14T (ex-type strain TBRC 11425=PYCC 8359=CGMCC 2.6179; Mycobank number 833394) and W. tropicalis DMKU VGT1-19T (ex-type strain TBRC 11426=PYCC 8360=CGMCC 2.6180; Mycobank number 833393).
Tremella ananatis sp. nov. and Tremella lamprococci sp. nov., two yeast species associated with bromeliads
Eight yeast isolates with an affinity to the genus Tremella were obtained from bromeliads from different locations in Brazil. Although the formation of basidia and basidiocarp were not observed on the basis of the results of sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region we suggest that these isolates represent two novel species of the genus Tremella. These yeasts are phylogenetically related to Tremella saccharicola and Tremella globispora. Therefore we propose Tremella ananatis sp. nov. and Tremella lamprococci sp. nov. as novel yeast species of the order Tremellales (Agaricomycotina Basidiomycota). Sequence analysis revealed that Tremella ananatis sp. nov. differs by 11 and 28 nucleotide substitutions from Tremella saccharicola in the D1/D2 sequence and ITS region respectively. Moreover Tremella lamprococci sp. nov. differs by 15 and 29 nucleotide substitutions from Tremella globispora in the D1/D2 sequence and ITS region respectively. The holotypes of Tremella ananatis sp. nov. and Tremella lamprococci sp. nov. are CBS 14568T and CBS 14567T and the MycoBank numbers are MB840480 and MB840481 respectively.
Pichia bovicola sp. nov., a yeast species associated with small-intestinal content of cattle
Three yeast strains DMKU-MP6-4T DMKU-MP2-6 and DMKU-MP5-1 were isolated from the small-intestinal content or Pia of cattle in Thailand during the investigation of yeast diversity in this habitat. According to the D1/D2 domain of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region sequence analysis these strains represent a novel yeast species in the genus Pichia. The species produced one to four ascospores per ascus with spherical to ellipsoidal shape and heterogenous in terms of size. These three strains were identical and differed from their closely related species Pichia exigua NRRL Y-10920T by 2% (six nucleotide substitutions and five gaps) in the D1/D2 domain of the large subunit rRNA gene while ITS region sequences differed by 3.1% (16 nucleotide substitutions and 27 gaps) 3.7% (19 nucleotide substitutions and 28 gaps) and 3.1% (16 nucleotide substitutions and 27 gaps) for DMKU-MP6-4T DMKU-MP2-6 and DMKU-MP5-1 respectively. The name Pichia bovicola is proposed to accommodate these species. The holotype is DMKU-MP6-4T (TBRC 15616T=PYCC 8905T).
Savitreea pentosicarens gen. nov., sp. nov., a yeast species in the family Saccharomycetaceae isolated from a grease trap
Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene the D1/D2 domain of the LSU rRNA gene the ITS region translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence Savitreea pentosicarens gen. nov. sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).
Cyberlindnera sylvatica sp. nov., a yeast species isolated from forest habitats
Five yeast strains isolated from forest habitats in Hungary and Germany were characterized phenotypically and by sequencing of the D1/D2 domain of the large subunit rRNA gene and the ITS1-5.8S-ITS2 (ITS) region of the rRNA gene. The strains have identical D1/D2 domain and ITS region sequences. By sequence comparisons Candida mycetangii and Candida maritima were identified as the closest relatives among the currently recognized yeast species. The DNA sequences of the investigated strains differ by 1.2 % (six substitutions) in the D1/D2 domain and by 3.5 % (12 substitutions and eight indels) in the ITS region from the type strain of C. mycetangii (CBS 8675T) while by 1.2 % (six substitutions and one indel) in the D1/D2 domain and by 7 % (32 substitutions and seven indels) in the ITS region from the type strain of C. maritima (CBS 5107T). Because the intraspecies heterogeneity seems to be very low and the distance to the most closely related species is above the commonly expected level for intraspecies variability Cyberlindnera sylvatica sp. nov. (holotype CBS 16335T; isotype NCAIM Y.02233T; MycoBank no. MB 835268) is proposed to accommodate the above-noted five yeast strains. Phenotypically the novel species can be distinguished from C. mycetangii and C. maritima by the formation of ascospores. Cyberlindnera sylvatica forms one or two hat-shaped ascospores per ascus on many different media as well as well-developed pseudohyphae and true hyphae. Additionally we propose the transfer of three anamorphic members of the Cyberlindnera americana sub-clade to the genus Cyberlindnera as the following new taxonomic combinations Cyberlindnera maritima f.a. comb. nov. Cyberlindnera mycetangii f.a. comb. nov. and Cyberlindnera nakhonratchasimensis f.a. comb. nov.
Magnusiomyces siamensis sp. nov., a yeast-like fungus isolated from food waste
Three yeast strains DMKU-GTSP8-6 DMKU-GTSP8-14T and DMKU-JED8-73 were isolated from food waste in Thailand. Based on the phenotypic characteristics and sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region it was revealed that the three strains clustered with the Magnusiomyces/Saprochaete clade. These strains were distinguished from the closely related species Saprochaete quercus CBS 750.85 Magnusiomyces ovetensis CBS 192.55T Magnusiomyces starmeri CBS 780.96T Saprochaete chiloensis CBS 8187T and Magnusiomyces ingens CBS 517.90T by 11.4 13.1 11.9 11.2 and 12.6 % sequence divergence in the D1/D2 domain and by 34.6 34.5 33.6 33.2 and 34.9 % sequence divergence in the ITS region respectively. The new species which does not produce ascospores is described as Magnusiomyces siamensis. The holotype of Magnusiomyces siamensis is TBRC 15056T and the isotypes are DMKU-GTSP8-14T and PYCC 9023T.
Diddensiella parasantjacobensis f.a., sp. nov., a yeast species from forest habitats
Six conspecific yeast strains representing an undescribed species were isolated from rotten wood collected in different locations in Hungary and Germany and an additional one from fungal fruiting body in Taiwan. The seven strains share identical nucleotide sequences in the D1/D2 domain of the nuclear large subunit (LSU) rRNA gene. The Hungarian and Taiwanese isolates share identical internal transcribed spacer (ITS) sequences as well while the two German isolates differ from them merely by three substitutions and four indels in this region. The investigated strains are very closely related to Diddensiella santjacobensis. Along their LSU D1/D2 domain they differ only by one substitution from the type strain of D. santjacobensis. However in the ITS region of Hungarian and Taiwanese strains we detected 3.5 % divergence (nine substitutions and nine indels) between the undescribed species and D. santjacobensis while the German strains differed by 13 substitutions and nine indels from D. santjacobensis. This ITS sequence divergence has raised the possibility that the strains investigated in this study may represent a different species from D. santjacobensis. This hypothesis was supported by comparisons of partial translation elongation factor 1-α (EF-1α) and cytochrome oxidase II (COX II) gene sequences. While no difference and 1–2 substitutions among the partial EF-1α and COX II gene sequences of the strains of the undescribed species respectively were detected; the undescribed species differ by about 4 % (36 substitutions) and 10 % (50–51 substitutions) from D. santjacobensis in these regions. Parsimony network analysis of the partial COX II gene sequences also separated the investigated strains from the type strain of D. santjacobensis. In this paper we propose Diddensiella parasantjacobensis f.a. sp. nov. (holotype: NCAIM Y.02121; isotypes: CBS 17819 DSM 114156) to accommodate the above-noted strains.