RESULTS:
1 - 10 of 10 for ""School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, PR China""
Hyphopichia xiaguanensis f.a., sp. nov., an ascomycetous yeast species isolated from plant leaves
Two strains representing a novel yeast species were isolated from plant leaves collected in the Baotianman Nature Reserve in Henan Province central China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and the D1/D2 domain of the large subunit rRNA gene revealed that the novel species belonged to the genus Hyphopichia although the formation of ascospores was not observed. The novel species was related most closely to Hyphopichia paragotoi CBS 13913T but they differed by 0.9 % sequence divergence (five substitutions) in the D1/D2 domain and by 3.7 % sequence divergence (seven substitutions and eight gaps) in the ITS region. Furthermore the novel species can also be differentiated from the closely related species in some biochemical and physiological characteristics. The species name of Hyphopichia xiaguanensis f.a. sp. nov. (Holotype CBS 16668 Mycobank MB 842425) is proposed to accommodate strains NYNU 20899T and NYNU 20914.
Torulaspora jiuxiensis sp. nov., a novel yeast species isolated from rotting wood
Two strains of a novel ascomycetous yeast species were isolated from rotting wood samples collected in Jiuxi Mountain Forest Park in Yunnan Province southwest China. Both strains formed one or two spherical ascospores in persistent asci. Phylogenetic analysis of the concatenated sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and the D1/D2 domain of the large subunit rRNA gene revealed that the novel strains represented a phylogenetically distinct species belonging to the genus Torulaspora. This novel species differed from the type strains of the closest known species Torulaspora nypae and Torulaspora maleeae by 0.9 and 1.2 % nucleotide substitutions in the D1/D2 domain and 5.3 and 6 % nucleotide substitutions in the ITS region respectively. The novel species can also be distinguished from T. nypae and T. maleeae in terms of the ability to assimilate ribitol succinate and citrate and its ability to grow at 37 °C. The species name of Torulaspora jiuxiensis sp. nov. is proposed with holotype CBS 16004T (Mycobank MB 844535).
Barnettozyma menglunensis f.a., sp. nov., a novel yeast species isolated from rotting wood
A novel yeast species is described based on three strains isolated from rotting wood samples from Xishuangbanna Tropical Rainforest in Yunnan Province PR China. Strain NYNU 1811121 was isolated in Menglun Mengla while strains NYNU 18982 and NYNU 181096 were recovered in Mengyang Jinghong. Analysis of the sequences of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) revealed that the novel strains were closely related to the type strain of [Candida] sanyiensis but with 6.9 % nucleotide substitutions in the D1/D2 domain and 8.2 % substitutions in the ITS region. The three novel strains can also be distinguished from C. sanyiensis in terms of the ability to assimilate trehalose and d-gluconate and to grow at 35 °C as well as the inability to ferment glucose. Based on molecular analyses and phenotypic characteristics the name Barnettozyma menglunensis f.a. sp. nov. is proposed with the holotype CBS 16011T (MycoBank 845375).
Hanseniaspora menglaensis f.a., sp. nov., a novel apiculate yeast species isolated from rotting wood
Two apiculate strains (NYNU 181072 and NYNU 181083) of a bipolar budding yeast species were isolated from rotting wood samples collected in Xishuangbanna Tropical Rainforest in Yunnan Province southwest PR China. On the basis of phenotypic characteristics and the results of phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA internal transcribed spacer (ITS) region and the actin (ACT1) gene the two strains were found to represent a single novel species of the genus Hanseniaspora for which the name Hanseniaspora menglaensis f.a. sp. nov. (holotype CICC 33364T; MycoBank MB 847437) is proposed. In the phylogenetic tree H. menglaensis sp. nov. showed a close relationship with Hanseniaspora lindneri Hanseniaspora mollemarum Hanseniaspora smithiae and Hanseniaspora valbyensis. H. menglaensis sp. nov. differed from H. lindneri the most closely related known species by 1.2 % substitutions in the D1/D2 domain 2.5 % substitutions in the ITS region and 5.4 % substitutions in the ACT1 gene respectively. Physiologically H. menglaensis sp. nov. can also be distinguished from H. lindneri by its ability to assimilate d-gluconate.
Danielozyma pruni sp. nov., an asexual yeast species isolated from insect frass
Two strains (NYNU 218101 and NYNU 218104) of an asexual yeast species were isolated from insect frass collected in insect tunnels of red leaf plum trees in the Henan Province central China. Molecular phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region showed that these two strains belonged to the genus Danielozyma with Danielozyma litseae as the closest known species. They differed from the type strain of D. litseae by 0.6 % substitutions (three substitutions and one gap) in the D1/D2 domain and by 5.1 % substitutions (19 substitutions and six gaps) in the ITS region respectively. When compared with the partial ACT1 TEF1 and RPB1 gene sequences they differed by 3 % (26 substitutions) 2.7 % (25 substitutions) and 9 %(54 substitutions) from D. litseae NRRL YB-3246T in these regions. Physiologically they also differed from its closest known species D. litseae based on the ability to assimilate inulin and galactitol as well as to grow in 0.1 % cycloheximide and its inability to ferment maltose and raffinose. In order to classify the two new isolates based on morphological and molecular evidence we proposed the description of a novel species Danielozyma pruni sp. nov. with strain JCM 35735T as holotype (Mycobank MB 849101).
Spencermartinsiella henanensis fa., sp. nov., a novel yeast species isolated from rotting wood
Two yeast strains (NYNU 211162 and NYNU 211275) were isolated from rotting wood collected in the Baotianman Nature Reserve Henan Province central China. Phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that the strains represent a phylogenetically distinct species within the genus Spencermartinsiella. The name Spencermartinsiella henanensis fa. sp. nov. is proposed for this species with holotype CICC 33543T (Mycobank MB 851142). S. henanensis sp. nov. differed by only 3 nt (~0.5 %) substitutions from the closest known species S. europaea NCAIM Y.01817T in the D1/D2 domain but by 33 nt (~6 %) substitutions 34 nt (~3.8 %) substitutions 30 nt (~5.6 %) substitutions and 75 nt (~9.9 %) substitutions in the ITS region and the partial TEF1 COXII and RPB2 genes. Additionally S. henanensis sp. nov. can be physiologically distinguished from S. europaea by its ability to assimilate inulin inability to assimilate ethylamine and cadaverine and incapability of growth at 30 °C.
Naohidea akebiae fa. sp. nov., an anamorphic basidiomycete yeast species
A novel basidiomycete yeast species represented by strain NYNU 2211328 was isolated from a leaf of Akebia trifoliata collected from the Baotianman Nature Reserve in Henan Province central China. Phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region suggested that this strain is closely related to Naohidea sebacea CBS 8477 exhibiting the similarity values of 96.5% and 91.3% in the D1/D2 domain and the ITS region respectively. Physiologically the novel strain differs from N. sebacea in its ability to assimilate inulin trehalose and d-arabinose its inability to assimilate dl-lactate and its incapability of growth at 30 °C. Both phenotypic and phylogenetic analyses indicated that the isolated strain represents a novel species in the genus Naohidea and the name Naohidea akebiae fa. sp. nov. (holotype: CICC 33584; MycoBank number: MB 855585) is proposed.
Pichia kurtzmaniana f.a. sp. nov., with the transfer of eight Candida species to Pichia
Three yeast strains belonging to the ascomycetous yeast genus Pichia were isolated from two soil samples from Yunnan and Guizhou provinces and a marine water sample from Liaoning province PR China. Phylogenetic analyses based on the sequences of the D1/D2 domains of the large subunit(LSU) rRNA gene and the internal transcribed spacer (ITS) region indicate that these three strains together with 12 additional strains isolated from various substrates collected in different regions or countries of the world represent a novel species of the genus Pichia for which the name Pichia kurtzmaniana sp. nov. (holotype: strain CGMCC 2.7213) is proposed. The novel species differs from its close relatives Candida californica by eight (1.5 %) and 26 (11.1 %) mismatches in the D1/D2 domains and the ITS region respectively; and from Pichia chibodasensis by 11 (2.1 %) and 20 (8.7 %) mismatches in the D1/D2 domains and the ITS region respectively. In addition eight Candida species which belong to the Pichia clade are transferred to the genus Pichia resulting in the proposal of the following new combinations: Pichia cabralensis comb. nov. Pichia californica comb. nov. Pichia ethanolica comb. nov. Pichia inconspicua comb. nov. Pichia phayaonensis comb. nov. Pichia pseudolambica comb. nov. Pichia rugopelliculosa comb. nov. and Pichia thaimueangensis comb. nov.
Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge
A novel bacterium designated BD-1T was isolated from a sludge sample. Cells of the novel Gram-stain-negative strain were identified to be facultative anaerobic non-motile and short rod-shaped. Growth occurred at 15–37 °C (optimum 30 °C) pH 5.0–10.0 (pH 7.0) and in 0–4.0 % NaCl (2.0 % w/v). The 16S rRNA gene sequence of strain BD-1T showed the highest sequence similarity to Ottowia thiooxydans DSM 14619T (97.0 %) followed by Ottowia pentelensis DSM 21699T (96.3 %) and less than 96 % to other related strains. The phylogenetic trees revealed that strain BD-1T clustered within the genus Ottowia . Summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c 48.2 %) C16 : 0 (23.2 %) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c 8.6 %) were the major fatty acids (>5 %) and ubiquinone-8 was the respiratory quinone. Phosphatidylethanolamine phosphatidylmethylethanolamine and phosphatidylglycerol were identified as the major polar lipids. Meanwhile the G+C content of the DNA was 63.6 mol% based on the draft genome analysis. The average nucleotide identity and digital DNA–DNA hybridization values between strain BD-1T and DSM 14619T were 74.5 and 21.4 % respectively. In addition the novel strain completely degraded 500 mg l−1 phenylacetic acid within 72 h under the condition of 3 % NaCl. Given the results of genomic phylogenetic phenotypic and chemotaxonomic analyses strain BD-1T was considered to represent a novel species of the genus Ottowia for which the name Ottowia caeni sp. nov. is proposed. The strain is a potential resource for the bioremediation of phenylacetic acid contaminated water. The type strain is BD-1T (=CGMCC 1.18541T=KCTC 82183T).
Saturnispora sinensis sp. nov., a new ascomycetous yeast species from soil and rotten wood
A yeast strain (CGMCC 2.6937T) belonging to the ascomycetous yeast genus Saturnispora was recently isolated from soil collected in Xinghuacun Shanxi Province PR China. The strain produces one or two ellipsoid or spherical ascospores in asci formed by the conjugation between a cell and its bud. Phylogenetic analyses of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit rRNA gene suggest that this strain is conspecific with strains NYNU 14639 isolated from rotten wood collected in Funiu Mountain Henan province and ES13S05 from soil collected in Nantou County Taiwan. The CGMCC 2.6937T group is most closely related to Saturnispora dispora and Saturnispora zaruensis. However strain CGMCC 2.6937T differs from S. dispora by 17 (3.2 % 13 substitutions and four gaps) and 77 (18.8 % 52 substitutions and 25 gaps) mismatches and from S. zaruensis by 15 (2.9 % 12 substitutions and three gaps) and 64 (15.6 % 44 substitutions and 20 gaps) mismatches in the D1/D2 domain and ITS region respectively. The results suggest that the CGMCC 2.6937T group represents an undescribed species in the genus Saturnispora for which the name Saturnispora sinensis sp. nov. is proposed. The holotype strain is CGMCC 2.6937T.