RESULTS:
1 - 4 of 4 for ""ascomycetous yeasts""
Allodekkera sacchari gen. nov., sp. nov., a yeast species in the Saccharomycetales isolated from a sugar factory
Three yeast isolates G5-5(5)T G5-9(3) and G5-9(4) were obtained from the sugar cane juice and waste from sugar production plant (Korach Industry Co. Ltd) in Korach province Thailand. They were found to belong to the same species based on DNA sequence identity of the small subunit ribosomal RNA gene (SSU) and the D1/D2 region of the large subunit rRNA gene (LSU D1/D2). A blastn search of the GenBank database revealed they had 93 % nucleotide sequence identity to Dekkera bruxellensis for the SSU (1742 bp) but their LSU D1/D2 sequence (572 bp) showed less than 90 % identity to all available sequences in the database. Phylogenetic analyses with neighbour-joining and maximum-parsimony methods using the aligned LSU D1/D2 and SSU sequences (a total of 2072 positions after removal of gaps) inferred that the three isolates were separated from all known taxa in the Saccharomycetales and that the neighbouring taxa were species of Dekkera/Brettanomyces. Physiological and biochemical characters revealed distinct differences between the three isolates and Dekkera/Brettanomyces species including the ability to assimilate several carbon sources and inability to ferment glucose. Thus isolates G5-5(5)T G5-9(3) and G5-9(4) should be assigned to a novel taxon for which the name Allodekkera sacchari gen. nov. sp. nov. is proposed. The type strain of the type species is G5-5(5)T (=CBS 14167T=JCM 18455T=TISTR 5950T) with MycoBank number MB815477 (for the genus) and MB817751 (for the species). Two additional strains of the species are G5-9(3) (=JCM 18456) and G5-9(4) (=JCM 18457).
Hanseniaspora terricola sp. nov., an ascomycetous yeast isolated from Tibet
Eight apiculate strains isolated from Tibet PR China were identified as Hanseniaspora taiwanica and a novel species of Hanseniaspora based on the sequence analysis of the ITS region the D1/D2 domains of the LSU rRNA and the translation elongation factor 1-a (TEF1) gene. Among them four strains with identical sequences of D1/D2 and ITS formed a separate branch from the known Hanseniaspora species in the phylogenetic trees and differed from the known species by at least 17 (3 %) nucleotide (nt) substitutions in the D1/D2 domains and more than 6 % substitutions and inserts/deletes in the ITS region. The phylogenetic analysis indicated that those four strains represent a novel species of Hanseniaspora for which the names Hanseniaspora terricola sp. nov. (holotype CGMCC 2.6175T; MycoBank MB 834591) is proposed. The other four strains belonging to H. taiwanica produce spherical void or fusiform ascospores which differ from the original description that ascospores are absent.
Barnettozyma menglunensis f.a., sp. nov., a novel yeast species isolated from rotting wood
A novel yeast species is described based on three strains isolated from rotting wood samples from Xishuangbanna Tropical Rainforest in Yunnan Province PR China. Strain NYNU 1811121 was isolated in Menglun Mengla while strains NYNU 18982 and NYNU 181096 were recovered in Mengyang Jinghong. Analysis of the sequences of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) revealed that the novel strains were closely related to the type strain of [Candida] sanyiensis but with 6.9 % nucleotide substitutions in the D1/D2 domain and 8.2 % substitutions in the ITS region. The three novel strains can also be distinguished from C. sanyiensis in terms of the ability to assimilate trehalose and d-gluconate and to grow at 35 °C as well as the inability to ferment glucose. Based on molecular analyses and phenotypic characteristics the name Barnettozyma menglunensis f.a. sp. nov. is proposed with the holotype CBS 16011T (MycoBank 845375).
Cyberlindnera qingyuanensis f.a., sp. nov., a yeast species isolated from rotting wood
Two yeast strains were isolated from rotting wood samples collected on Qingyuan Mountain Fujian Province PR China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit rRNA gene revealed that these two strains represent a novel species of the genus Cyberlindnera. The proposed name for this new species is Cyberlindnera qingyuanensis f.a. sp. nov. (holotype: GDMCC 2.300; ex-type: PYCC 9925) although the formation of ascospores was not observed. The novel species differs from its close relative Cyberlindnera galapagoensis by 7.7% sequence divergence (37 substitutions and seven indels) in the D1/D2 domain and 9.7% sequence divergence (42 substitutions and 34 indels) in the ITS region respectively. Additionally Cyb. qingyuanensis differs from its close relative Cyb. galapagoensis by its ability to grow in cellobiose l-rhamnose ribitol galactitol and dl-lactate its growth at 37 °C and its inability to ferment raffinose. The Mycobank number is MB 854693.