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Metahyphopichia suwanaadthiae sp. nov., an anamorphic yeast species in the order Saccharomycetales and reassignment of Candida silvanorum to the genus Metahyphopichia
Seven yeast strains representing a single novel anamorphic species were isolated in Thailand. They consisted of five strains (DMKU-MRY16T DMKU-SK18 DMKU-SK25 DMKU-SK30 and DMKU-SK32) obtained from five different mushrooms and two strains (ST-224 and 11-14.2) derived from insect frass and soil respectively. The pairwise sequence analysis indicated that all seven strains had identical sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. Metahyphopichia silvanorum was the most closely related species but with 11.9–12.4% nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 13.1–13.3% nucleotide substitutions in the ITS region. The phylogenetic analyses based on the concatenated sequences of the ITS region and the D1/D2 domains of the LSU rRNA gene showed that the seven strains form a well-separated subclade in a clade containing M. silvanorum and Metahyphopichia laotica with high bootstrap support. A phylogenetic analysis of a multilocus dataset including the small subunit (SSU) rRNA gene the ITS region the D1/D2 domains of the LSU rRNA gene translation elongation factor 1-alpha gene actin gene and the RNA polymerase II subunit 2 gene confirmed the presence of the monophyletic clade that also includes M. silvanorum and M. laotica and strongly supported the phylogenetic isolation of the seven strains from its neighbouring species. Therefore the seven strains were assigned as a single novel species of the genus Metahyphopichia according to their phylogenetic relationships. The name Metahyphopichia suwanaadthiae sp. nov. is proposed to accommodate the seven strains. The holotype is DMKU-MRY16T (TBRC 11775T=NBRC 114386T=PYCC 8655T). The MycoBank number of the novel species is MB 841280. In addition Candida silvanorum is reassigned to the genus Metahyphopichia. The MycoBank number of M. silvanorum comb. nov. is MB 841279.
Danielozyma pruni sp. nov., an asexual yeast species isolated from insect frass
Two strains (NYNU 218101 and NYNU 218104) of an asexual yeast species were isolated from insect frass collected in insect tunnels of red leaf plum trees in the Henan Province central China. Molecular phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region showed that these two strains belonged to the genus Danielozyma with Danielozyma litseae as the closest known species. They differed from the type strain of D. litseae by 0.6 % substitutions (three substitutions and one gap) in the D1/D2 domain and by 5.1 % substitutions (19 substitutions and six gaps) in the ITS region respectively. When compared with the partial ACT1 TEF1 and RPB1 gene sequences they differed by 3 % (26 substitutions) 2.7 % (25 substitutions) and 9 %(54 substitutions) from D. litseae NRRL YB-3246T in these regions. Physiologically they also differed from its closest known species D. litseae based on the ability to assimilate inulin and galactitol as well as to grow in 0.1 % cycloheximide and its inability to ferment maltose and raffinose. In order to classify the two new isolates based on morphological and molecular evidence we proposed the description of a novel species Danielozyma pruni sp. nov. with strain JCM 35735T as holotype (Mycobank MB 849101).