RESULTS:
1 - 10 of 10 for ""College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, PR China""
Ktedonosporobacter rubrisoli gen. nov., sp. nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam. nov.
A novel filamentous spore-forming Gram-stain-positive bacterium designated SCAWS-G2T was isolated from red soil in Jiangxi Province PR China. The strain grew at 25–45 °C and at pH 4.0–7.0 and was able to tolerate up to 50 mM Zn2+. The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb which contained four 16S rRNA genes with three sequence types (0.4–0.8 % differences). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales showing <89.2 % sequence similarities to the recognized taxa of this order. The whole-genome based phylogenomic tree separated strain SCAWS-G2T from the recognized families within Ktedonobacterales . The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %. The strain can also be differentiated from the recognized families by a number of phenotypic characteristics. The polar lipids of SCAWS-G2T were diphosphatidylglycerol phosphatidylglycerol phosphatidylinositol seven unidentified glycolipids and one unidentified lipid. The peptidoglycan amino acids contained ornithine glycine glutamic acid and alanine and the cell-wall sugars were mainly galactose and rhamnose. The major fatty acids were C16 : 1 2-OH C16 : 0 and iso-C17 : 0. Based on all these data we propose that strain SCAWS-G2T represents a novel genus and species Ktedonosporobacter rubrisoli gen. nov. sp. nov. within the new family Ktedonosporobacteraceae fam. nov. of the order Ktedonobacterales . The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
Lentzea tibetensis sp. nov., a novel Actinobacterium with antimicrobial activity isolated from soil of the Qinghai–Tibet Plateau
A novel filamentous Actinobacterium designated strain FXJ1.1311T was isolated from soil collected in Ngari (Ali) Prefecture Qinghai-Tibet Plateau western PR China. The strain showed antimicrobial activity against Gram-positive bacteria and Fusarium oxysporum. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FXJ1.1311T belonged to the genus Lentzea and showed the highest sequence similarity to Lentzea guizhouensis DHS C013T (98.04%). Morphological and chemotaxonomic characteristics supported its assignment to the genus Lentzea . The genome-wide average nucleotide identity between strain FXJ1.1311T and L. guizhouensis DHS C013T as well as other Lentzea type strains was <82.2 %. Strain FXJ1.1311T also formed a monophyletic line distinct from the known Lentzea species in the phylogenomic tree. In addition physiological and chemotaxonomic characteristics allowed phenotypic differentiation of the novel strain from L. guizhouensis . Based on the evidence presented here strain FXJ1.1311T represents a novel species of the genus Lentzea for which the name Lentzea tibetensis sp. nov. is proposed. The type strain is FXJ1.1311T (=CGMCC 4.7383T=DSM 104975T).
Pichia kurtzmaniana f.a. sp. nov., with the transfer of eight Candida species to Pichia
Three yeast strains belonging to the ascomycetous yeast genus Pichia were isolated from two soil samples from Yunnan and Guizhou provinces and a marine water sample from Liaoning province PR China. Phylogenetic analyses based on the sequences of the D1/D2 domains of the large subunit(LSU) rRNA gene and the internal transcribed spacer (ITS) region indicate that these three strains together with 12 additional strains isolated from various substrates collected in different regions or countries of the world represent a novel species of the genus Pichia for which the name Pichia kurtzmaniana sp. nov. (holotype: strain CGMCC 2.7213) is proposed. The novel species differs from its close relatives Candida californica by eight (1.5 %) and 26 (11.1 %) mismatches in the D1/D2 domains and the ITS region respectively; and from Pichia chibodasensis by 11 (2.1 %) and 20 (8.7 %) mismatches in the D1/D2 domains and the ITS region respectively. In addition eight Candida species which belong to the Pichia clade are transferred to the genus Pichia resulting in the proposal of the following new combinations: Pichia cabralensis comb. nov. Pichia californica comb. nov. Pichia ethanolica comb. nov. Pichia inconspicua comb. nov. Pichia phayaonensis comb. nov. Pichia pseudolambica comb. nov. Pichia rugopelliculosa comb. nov. and Pichia thaimueangensis comb. nov.
ICTV Virus Taxonomy Profile: Ovaliviridae
Ovaliviridae is a family of enveloped viruses with a linear dsDNA genome. The virions are ellipsoidal and contain a multi-layered spool-like capsid. The viral genome is presumably replicated through protein priming by a putative DNA polymerase encoded by the virus. Progeny virions are released through hexagonal openings resulting from the rupture of virus-associated pyramids formed on the surface of infected cells. The only known host is a hyperthermophilic archaeon of the genus Sulfolobus . This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Ovaliviridae which is available at ictv.global/report/ovaliviridae.
Vishniacozyma pseudocarnescens sp. nov., a new anamorphic tremellomycetous yeast species
Three strains belonging to the basidiomycetous yeast genus Vishniacozyma were isolated from marine water samples collected from intertidal zones in Liaoning province northeast China. Phylogenetic analyses based on the sequences of the small subunit (SSU) ribosomal DNA (rDNA) the D1/D2 domain of the large subunit (LSU) ribosomal DNA (rDNA) the internal transcribed spacer region (ITS) the two subunits of DNA polymerase II (RPB1 and RPB2) the translation elongation factor 1-α (TEF1) and the mitochondrial gene cytochrome b (CYTB) showed that these strains together with 20 strains from various geographic and ecological origins from other regions of the world represent a novel species in the genus Vishniacozyma. We propose the name Vishniacozyma pseudocarnescens sp. nov. (holotype CGMCC 2.6457) for the new species which differs phenotypically from its close relatives V. carnescens V. tephrensis and V. victoriae by its ability to grow at 30 °C and on 50 % (w/v) glucose-yeast extract agar.
Cystofilobasidium josepaulonis sp. nov., a novel basidiomycetous yeast species
A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province PR China. The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain together with three other strains isolated from basal ice collected in Norway the gut of an insect and an alga collected in Russia represent a novel species of the genus for which the name Cystofilobasidium josepaulonis sp. nov. (holotype strain CGMCC 2.6672T) is proposed. The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %–4.1 and 11.3 %–17.1 % mismatches in the D1/D2 domain and the ITS region respectively. This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar but teliospore germination with basidia was not observed.
Saturnispora sinensis sp. nov., a new ascomycetous yeast species from soil and rotten wood
A yeast strain (CGMCC 2.6937T) belonging to the ascomycetous yeast genus Saturnispora was recently isolated from soil collected in Xinghuacun Shanxi Province PR China. The strain produces one or two ellipsoid or spherical ascospores in asci formed by the conjugation between a cell and its bud. Phylogenetic analyses of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit rRNA gene suggest that this strain is conspecific with strains NYNU 14639 isolated from rotten wood collected in Funiu Mountain Henan province and ES13S05 from soil collected in Nantou County Taiwan. The CGMCC 2.6937T group is most closely related to Saturnispora dispora and Saturnispora zaruensis. However strain CGMCC 2.6937T differs from S. dispora by 17 (3.2 % 13 substitutions and four gaps) and 77 (18.8 % 52 substitutions and 25 gaps) mismatches and from S. zaruensis by 15 (2.9 % 12 substitutions and three gaps) and 64 (15.6 % 44 substitutions and 20 gaps) mismatches in the D1/D2 domain and ITS region respectively. The results suggest that the CGMCC 2.6937T group represents an undescribed species in the genus Saturnispora for which the name Saturnispora sinensis sp. nov. is proposed. The holotype strain is CGMCC 2.6937T.
Gimesia benthica sp. nov., a planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean
A Gram-stain-negative stalked oval-shaped and budding bacterial strain designated E7T was isolated from a deep-sea water sample collected from the Northwest Indian Ocean. The novel strain was strictly aerobic and catalase- and oxidase-positive. It grew at 6–40 °C (optimum 30 °C) and pH 5.5–8.0 (optimum pH 7.0–7.5). The strain required 0.5–9.0 % (w/v) NaCl (optimum 3.0–5.0 %) for growth. Aesculin starch pectin and Tween 20 were hydrolysed. Based on 16S rRNA gene sequence analysis strain E7T showed the highest similarity with Gimesia maris DSM 8797T (97.5 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain E7T and G. maris DSM 8797T were 78.0 and 19.3 % respectively. The predominant cellular fatty acids of strain E7T were C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine (PE) phosphatidylmonomethylethanolamine (PMME) phosphatidyldimethylethanolamine (PDME) phosphatidylcholine (PC) and diphosphatidylglycerol (DPG). The genomic DNA G+C content of strain E7T was 52.8 mol%. On the basis of phylogenetic inference and phenotypic characteristics it is proposed that strain E7T represents a novel species of the genus Gimesia for which the name Gimesia benthica sp. nov. is proposed. The type strain is E7T (=CGMCC 1.16119T=KCTC 72737T).
Starmerella fangiana f.a. sp. nov., a new ascomycetous yeast species from Daqu-making environment and other sources
In the survey of yeast diversity in high-temperature Daqu which is a fermentation starter for Chinese sauce-flavoured Baijiu six yeast strains representing one novel species of the genus Starmerella were isolated from samples of Daqu and surrounding environments collected in Zunyi city Guizhou Province China. Phylogenetic analyses of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene indicate that these six strains are conspecific with three other strains isolated from flowers and duckweed collected in Samoa India and Thailand. The representative strain QFC-8 of the new species differs from the closet species Starmerella caucasica resolved by the D1/D2 sequence analysis by 13 (3.1 % 12 substitutions and 1 gap) and 40 (10.3 % 9 substitutions and 31 gaps) mismatches in the D1/D2 domain and ITS region respectively. The results suggest that the novel group represents an undescribed species in the genus Starmerella for which the name Starmerella fangiana sp. nov. is proposed. The holotype strain is CGMCC 2.7773.
Halovulum marinum sp. nov., isolated from deep-sea water of the Indian Ocean, and emended description of the genus Halovulum
A novel Gram-stain-negative aerobic motile by peritrichous flagella oval to rod-shaped bacterium designated strain 2CG4T was isolated from a deep-sea water sample collected from the Northwest Indian Ocean. The results of phylogenetic analysis of both 16S rRNA gene and RpoC protein sequences indicated that this strain was affiliated with the genus Halovulum in the Amaricoccus clade of the family Rhodobacteraceae of the class Alphaproteobacteria sharing 95.3 % similarity at the 16S rRNA gene sequence level with the type strain of Halovulum dunhuangense YYQ-30T the only species in the genus Halovulum . The predominant fatty acids (>10 %) of 2CG4T were summed feature 8 (C18 : 1ω7c and/ or C18 : 1ω6c; 61.1 %) and cyclo-C19 : 0ω8c (15.6 %). The polar lipids of 2CG4T were phosphatidylethanolamine phosphatidylmethylethanolamine phosphatidylglycerol diphosphatidylglycerol phosphatidylcholine and sulfoquinovosyldiacylglycerol. The only isoprenoid quinone of 2CG4T was ubiquinone-10. The DNA G+C content of 2CG4T was determined to be 69.4 %. The central gene pufLM for the photosynthetic reaction was not detected. No growth occurred for 2CG4T in the absence of NaCl. On the basis of these data it is concluded that the 2CG4T represents a novel species of the genus Halovulum for which the name Halovulum marinum sp. nov. is proposed. The type strain is 2CG4T (=CGMCC 1.16468T=JCM 32611T).