David Rowlands collection
Each year, the Microbiology Society Council offer Honorary Membership to distinguished microbiologists who have made a significant contribution to the science. In 2019, David J. Rowlands (Emeritus Professor of Virology, University of Leeds) was appointed an Honorary Member.
This collection brings together Journal of General Virology articles authored by David Rowlands.
Collection Contents
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Proliferative Responses of T cells Primed Against Human Rhinovirus to other Rhinovirus Serotypes
More LessLymphocytes from mice immunized with human rhinovirus (HRV) serotypes 1A or 15 proliferated in vitro in response to HRV and the activated cells were shown to be helper T (Th) cells. Lymphocytes from mice primed with HRV-1A responded to seven of eight heterologous virus serotypes, the responses to other minor cell receptor group viruses being greater than to those belonging to the major cell receptor group. A similar bias was seen with cells from mice primed with HRV-15 in that they responded preferentially to other major receptor group viruses. This pattern of cross-serotype recognition was shown to be similar in three inbred mouse strains and was not dependent upon the major histocompatibility complex haplotype. These results have revealed that there are determinants within the viral proteins of a number of serotypes of HRV that are recognized by Th cells primed against a single HRV serotype. Thus, at the level of Th cell recognition of HRV, a cross-serotype reactivity is seen which is not reflected in the B cell antibody response to virus, which is generally highly serotype-specific.
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Parameters influencing the attachment of hepatitis A virus to a variety of continuous cell lines
More LessWe have investigated the interactions of purified radiolabelled hepatitis A virus (HAV) with a variety of continuous cell lines. Virus labelled either in vitro with radiolabelled iodine or in vivo with radiolabelled uridine bound to cells with similar efficiency. Attachment to BS-C-1 cells was calcium ion-dependent and this correlated with infectivity assay results. The cell tropism of HAV attachment was examined using cell suspensions and confluent cell monolayers at both 4 °C and 37 °C. The maximum level of attachment was observed at 4 °C with cells in suspension, but was severely inhibited by 2% foetal calf serum; these results again correlated with infectivity assays. The components of serum which inhibit attachment have been characterized by gel filtration chromatography, sucrose density gradient analysis, immunoprecipitation and Western blotting. The data show that such components are of high M r and that the serum glycoprotein, α2-macroglobulin, can partly mimic the inhibitory effect of whole serum.
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Presentation and Immunogenicity of Viral Epitopes on the Surface of Hybrid Hepatitis B Virus Core Particles Produced in Bacteria
More LessWe recently reported the enhanced immunogenicity of a peptide epitope when it was presented as a fusion protein with hepatitis B core antigen. In those experiments the fusion protein was expressed in vaccinia virus. We have now refined the system so that large amounts of highly immunogenic particles can be produced using a simple bacterial expression system. We describe the expression of three different viral epitopes as chimeric particles that induce good antibody responses to each epitope after one dose of low amounts of antigen. Finally we demonstrate that the immunogenicity is a reflection of both T helper cell sites within the core protein and also the particulate nature of the immunogens.
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A Physico-chemical Sub-grouping of the Mammalian Picornaviruses
More LessSummarySeveral of the physico-chemical properties of representative members of the Picornaviridae family have been examined. On the basis of their buoyant density in caesium chloride, stability at pH 3 to 7 and the base composition of the virus RNA, a division of this family of viruses into six subgroups is suggested.
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